Simultaneous Determination of Glutamate, Glycine, and Alanine in Human Plasma Using Precolumn Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate and High-Performance Liquid Chromatography

被引：11

作者：

Li, Qing Zhong ^{[1
,2
]}

Huang, Qing Xian ^{[3
]}

Li, Shu Cui ^{[1
,2
]}

Yang, Mei Zi ^{[1
,2
]}

Rao, Bin ^{[4
]}

机构：

[1] Binzhou Med Univ, Sch Pharm, Inst Pharm, Yantai 264003, Peoples R China

[2] Binzhou Med Univ, Sch Pharm, Dept Pharmacol, Yantai 264003, Peoples R China

[3] Yantai Yuhuangding Hosp, Dept Gen Surg, Yantai 264003, Peoples R China

[4] Hubei Prov Bafeng Pharmaceut & Chem Share Co Ltd, Dept Qual Inspect, Hefeng 445800, Peoples R China

来源：

KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | 2012年 / 16卷 / 05期

关键词：

Amino acids; AQC; HPLC; Plasma; Ultraviolet detection; ION-EXCHANGE CHROMATOGRAPHY; AMINO-ACID-ANALYSIS; REAGENT; URINE;

D O I：

10.4196/kjpp.2012.16.5.355

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A simple, sensitive and reproducible high-performance liquid chromatography (H PLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.

引用

页码：355 / 360

页数：6