Effective degradation of chicken feather waste by keratinase enzyme with triton X-100 additive

被引：3

作者：

Babbar, Nainpriya ^{[1
]}

Sharma, Geetika ^{[1
]}

Arya, Shailendra Kumar ^{[1
]}

机构：

[1] Panjab Univ, Univ Inst Engn & Technol, Dept Biotechnol, Chandigarh, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2022年 / 44卷

关键词：

Keratinase; Chicken feathers; Triton X-100; Keratin degradation; DECOMPOSITION;

D O I：

10.1016/j.bcab.2022.102447

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Keratinases are a group of proteolytic enzymes which play a crucial role in keratin degradation by breaking the disulfide and peptide bonds of the polypeptide chain of keratin. This study aimed to evaluate the biochemical characteristics of Keratinase enzyme produced from Bacillus licheniformis. The preliminary experimentation was performed in order to find the optimal conditions in which the enzyme showed maximum activity, and also to evaluate its residual activity by using activators and inhibitors. The maximum enzyme yield was 100%, observed at temperature 55 degrees C and pH 9, and the addition of Triton X-100 markedly enhanced the keratinase activity to 174%. Further on, it was observed that the enzyme activity first increased with the increasing concentrations of Triton-X 100, with maximum residual activity of 184 +/- 0.2% at 0.4 (v/v) Triton-X 100 concentration, and then experienced a downfall by further increase in the surfactant concentration, as it came down to 130 +/- 0.7% at 0.5 (v/v) concentration of Triton-X 100. The keratinase was finally utilized for the treatment of chicken feathers, and its keratinolytic activity was determined using SEM analysis.

引用

页数：7