Overexpression of an aquaglyceroporin gene from Trichoderma harzianum improves water-use efficiency and drought tolerance in Nicotiana tabacum

被引：14

作者：

Vieira, Pabline Marinho ^{[1
]}

Santos, Mirella Pupo ^{[2
]}

Andrade, Cristiana Moura ^{[3
,5
]}

Souza-Neto, Otacilio Antonio ^{[3
]}

Ulhoa, Cirano Jose ^{[4
]}

Lima Aragao, Francisco Jose ^{[3
]}

机构：

[1] Inst Fed Goiano, Dept Ciencias Biol, Lab Biotecnol, BR-75790000 Urutai, Go, Brazil

[2] Univ Fed Rio de Janeiro, Nupem, Lab Biotecnol Vegetal, BR-27910970 Macae, RJ, Brazil

[3] Embrapa Recursos Genet & Biotecnol, PqEB W5 Norte, BR-70770900 Brasilia, DF, Brazil

[4] Univ Fed Goias, Inst Ciencias Biol, Dept Bioquim & Biol Mol, Campus Samambaia,POB 131, BR-74001970 Goiania, Go, Brazil

[5] Dow AgroSci Brasil, Rod Anhanguera,Km 296, BR-14140000 Cravinhos, SP, Brazil

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2017年 / 121卷

关键词：

Abiotic stress tolerance; Aquaglyceroporin; Drought stress; Fungal gene; Transgenic plants; PLASMA-MEMBRANE AQUAPORINS; ROOT HYDRAULIC CONDUCTANCE; MESOPHYLL CONDUCTANCE; ARABIDOPSIS-THALIANA; PHOTOSYNTHESIS; EXPRESSION; TOBACCO; PLANTS; STRESS; CO2;

D O I：

10.1016/j.plaphy.2017.10.012

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aquaporins (AQPs) and aquaglyceroporins (AQGPs) are integral membrane proteins that mediate the transport of water and solutes, such as glycerol and urea, across membranes. AQP and AQGP genes represent a valuable tool for biotechnological improvement of plant tolerance to environmental stresses. We previously isolated a gene encoding for an aquaglyceroporin (ThAQGP), which was up-regulated in Trichoderma harzianum during interaction with the plant pathogen Fusarium solani. This gene was introduced into Nicotiana tabacum and plants were physiologically characterized. Under favorable growth conditions, transgenic progenies did not had differences in both germination and growth rates when compared to wild type. However, physiological responses under drought stress revealed that transgenic plants presented significantly higher transpiration rate, stomatal conductance, photosynthetic efficiency and faster turgor recovery than wild type. Quantitative RT-PCR analysis demonstrated the presence of ThAQGP transcripts in transgenic lines, showing the cause effect relationship between the observed phenotype and the expression of the transgene. Our results underscore the high potential of T. harzianum as a source of genes with promising applications in transgenic plants tolerant to drought stress.

引用

页码：38 / 47

页数：10