Modulation of Anti-Tumor Necrosis Factor Alpha (TNF-α) Antibody Secretion in Mice Immunized with TNF-α Kinoid

被引：13

作者：

Assier, Eric ^{[1
]}

Semerano, Luca ^{[1
,2
]}

Duvallet, Emilie ^{[1
]}

Delavallee, Laure ^{[1
]}

Bernier, Emilie ^{[3
]}

Laborie, Marion ^{[3
]}

Grouard-Vogel, Geraldine ^{[3
]}

Larcier, Patrick ^{[3
]}

Bessis, Natacha ^{[1
]}

Boissier, Marie-Christophe ^{[1
,2
]}

机构：

[1] Cite Univ Paris, Bobigny, France

[2] Grp Hosp Avicenne Jean Verdier Rene Muret, AP HP, Serv Rhumatol, Bobigny, France

[3] Neovacs SA, Paris, France

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2012年 / 19卷 / 05期

关键词：

RHEUMATOID-ARTHRITIS; CELL; METHOTREXATE; VACCINATION; MODEL; INHIBITION; INFLIXIMAB; THERAPY; AGENTS;

D O I：

10.1128/CVI.05649-11

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tumor necrosis factor alpha (TNF-alpha) blockade is an effective treatment for patients with TNF-alpha-dependent chronic inflammatory diseases, such as rheumatoid arthritis, Crohn's disease, and psoriasis. TNF-alpha kinoid, a heterocomplex of human TNF-alpha and keyhole limpet hemocyanin (KLH) (TNF-K), is an active immunotherapy targeting TNF-alpha. Since the TNF-K approach is an active immunization, and patients receiving this therapy also receive immunosuppressant treatment, we evaluated the effect of some immunosuppressive drugs on the generation of anti-TNF-alpha antibodies produced during TNF-K treatment. BALB/c mice were injected intramuscularly with TNF-K in ISA 51 adjuvant. Mice were also injected intraperitoneally with one of the following: phosphate-buffered saline, cyclophosphamide, methylprednisolone, or methotrexate. Anti-TNF-alpha and anti-KLH antibody levels were assessed by enzyme-linked immunosorbent assay and the anti-TNF-alpha neutralizing capacity of sera by L929 bioassay. Our results showed that current treatments used in rheumatoid arthritis, such as methylprednisolone and methotrexate, do not significantly alter anti-TNF-alpha antibody production after TNF-K immunization. In contrast, the administration of cyclophosphamide (200 mg/kg) after immunization significantly reduced anti-TNF-alpha antibody titers and their neutralizing capacity.

引用

页码：699 / 703

页数：5

共 30 条

[11] Proteasome inhibition for antibody-mediated rejection [J].