Meiotic cohesin complexes are essential for the formation of the axial element in mice

被引：94

作者：

Llano, Elena ^{[1
,2
]}

Herran, Yurema ^{[1
]}

Garcia-Tunon, Ignacio ^{[1
]}

Gutierrez-Caballero, Cristina ^{[1
]}

de Alava, Enrique ^{[1
]}

Luis Barbero, Jose ^{[4
]}

Schimenti, John ^{[5
]}

de Rooij, Dirk G. ^{[6
]}

Sanchez-Martin, Manuel ^{[3
]}

Pendas, Alberto M. ^{[1
]}

机构：

[1] Univ Salamanca, Inst Biol Mol & Celular Canc, CSIC, Salamanca 37007, Spain

[2] Univ Salamanca, Dept Fisiol, Consejo Super Invest Cient, Salamanca 37007, Spain

[3] Univ Salamanca, Dept Med, Consejo Super Invest Cient, Salamanca 37007, Spain

[4] CSIC, Dept Proliferac Celular & Desarrollo, Ctr Invest Biol, Madrid 28040, Spain

[5] Cornell Univ, Ctr Vertebrate Genom, Ithaca, NY 14850 USA

[6] Univ Amsterdam, Acad Med Ctr, Ctr Reprod Med, NL-1105 AZ Amsterdam, Netherlands

来源：

JOURNAL OF CELL BIOLOGY | 2012年 / 197卷 / 07期

关键词：

SISTER-CHROMATID COHESION; DOUBLE-STRAND BREAKS; SYNAPTONEMAL COMPLEX; CHROMOSOME SYNAPSIS; RECOMBINATION PROTEINS; MEIOSIS; SMC1-BETA; REPAIR; SYCP2; REC8;

D O I：

10.1083/jcb.201201100

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cohesin is a conserved multisubunit protein complex that participates in chromosome segregation, DNA damage repair, chromatin regulation, and synaptonemal complex (SC) formation. Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of the SC, suggesting that, in mammals, this function is not conserved. In this paper, we show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51-but not DMC1-mediated double-strand break repair, were not able to assemble their AEs, and arrested as early as the leptotene stage of prophase I, demonstrating that cohesin plays an essential role in AE assembly that is conserved from yeast to mammals.

引用

页码：877 / 885

页数：9

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