PEGylated Multi-Walled Carbon Nanotubes for Encapsulation and Sustained Release of Oxaliplatin

被引：78
作者：
Wu, Linlin [1 ,2 ]
Man, Changjun [3 ]
Wang, Hong
Lu, Xiaohe [4 ]
Ma, Qinghai [1 ,2 ]
Cai, Yu [5 ]
Ma, Wanshan [1 ,2 ]
机构：
[1] Shandong Prov Qianfoshan Hosp, Dept Lab Med, Jinan 250014, Peoples R China
[2] Shandong Univ, Jinan 250014, Peoples R China
[3] Jining Hosp Infect Dis, Dept Clin Lab, Jining 272031, Peoples R China
[4] Shandong Prov Qianfoshan Hosp, Shandong Acad Med Sci, Jinan 250014, Peoples R China
[5] Jinan Univ, Sch Pharm, Guangzhou 510632, Guangdong, Peoples R China
来源：
PHARMACEUTICAL RESEARCH | 2013年 / 30卷 / 02期
关键词：
carbon nanotube; cytotoxicity; HT29 cell line; oxaliplatin; PEGylation; HISTONE H2AX PHOSPHORYLATION; DOUBLE-STRAND BREAKS; CISPLATIN-DNA; IN-VITRO; RECOGNITION; COMBINATION;
D O I：
10.1007/s11095-012-0883-5
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
To develop PEGylated multi-walled carbon nanotubes as a sustained release drug delivery system. Oxaliplatin was incorporated into inner cavity of PEGylated multi-walled carbon nanotubes (MWCNT-PEG) using nano-extraction. Oxaliplatin release rates from MWCNT-PEG-Oxaliplatin were investigated using dialysis tubing. Cytotoxicity of oxaliplatin, MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin were evaluated in HT29 cell by MTT assay, Pt-DNA adducts formation, gamma-H2AX formation and cell apoptosis assay. Loading of oxaliplatin into MWCNT-PEG was similar to 43.6%. Sustained release occurred to MWCNT-PEG-Oxaliplatin, with only 34% of oxaliplatin released into medium within 6 h. In MTT assay, MWCNT-PEG-Oxaliplatin showed slightly decreased cytotoxic effect when cell viability was assessed at 12 and 24 h. A drastic increase of cytotoxicity was found when cell viability was assessed at 48 and 96 h. Pt-DNA adducts formation, gamma-H2AX formation and cell apoptosis assay results showed the same trend as the MTT assay, suggesting sustained-release for MWCNT-Oxaliplatin and MWCNT-PEG-Oxaliplatin formulations. PEGylated multi-walled carbon nanotubes can be used as sustained release drug delivery system, thus remarkably improving cytotoxicity of oxaliplatin on HT-29 cells.
引用
收藏
页码：412 / 423
页数：12
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