Noninvasive Stratum Corneum Sampling and Electron Microscopical Examination of Skin Barrier Integrity: Pilot Study with a Topical Glycerin Formulation for Atopic Dermatitis

被引:33
作者
Daehnhardt-Pfeiffer, S. [1 ]
Surber, C. [2 ,3 ]
Wilhelm, K-P [5 ]
Daehnhardt, D. [1 ]
Springmann, G. [5 ]
Boettcher, M. [1 ]
Foelster-Holst, R. [4 ]
机构
[1] Microscopy Serv Daehnhardt GmbH, DE-24220 Flintbek, Germany
[2] Dermatol Univ Klin, Basel, Switzerland
[3] Spirig Pharma AG, Egerkingen, Switzerland
[4] Univ Hautklin Kiel, Klin Dermatol Allergol & Venerol, Kiel, Germany
[5] ProDERM GmbH, Schenefeld Hamburg, Germany
关键词
Transmission electron microscopy; Emollients; Lipbarvis (R); Stratum corneum; Noninvasive skin sampling; Glycerin; Intercellular lipid lamellae; MOISTURIZER; EFFICACY;
D O I
10.1159/000336789
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Introduction: Therapy of atopic dermatitis encloses use of medicated and nonmedicated preparations. Results of clinical and biophysical procedures indirectly describe the condition of the impaired skin barrier (SB). Direct evaluation of SB integrity is only possible by electron microscopical visualization, e.g. intercellular lipid lamellae (ICLL) organization of the stratum corneum. Method: SB integrity was measured by morphometric analysis of ICLL in healthy and atopic skin and after a 15-day treatment (plus 7-day follow-up) of atopic skin with a glycerin preparation. Results: Significant treatment effect was shown by the restoration of the ICLL. Conclusions: The study reveals that morphometric analysis of ICLL organization is suitable to differentiate between healthy and diseased skin and to semiquantitatively determine the effect of a nonmedicated glycerin formulation. Limitation: Small treatment cohort. Copyright (C) 2012 S. Karger AG, Basel
引用
收藏
页码:155 / 161
页数:7
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