Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors

被引:30
作者
Jaremicz, Zbigniew [1 ]
Luczkiewicz, Maria [1 ]
Kokotkiewicz, Adam [1 ]
Krolicka, Aleksandra [2 ,3 ]
Sowinski, Pawel [4 ]
机构
[1] Med Acad Gdansk, Fac Pharm, Dept Pharmacognosy, PL-80416 Gdansk, Poland
[2] Univ Gdansk, Intercollegiate Fac Biotechnol, Dept Biotechnol, Lab Biol Act Cpds, PL-80822 Gdansk, Poland
[3] Med Univ Gdansk, PL-80822 Gdansk, Poland
[4] Gdansk Univ Technol, Fac Chem, Nucl Magnet Resonance Lab, PL-80233 Gdansk, Poland
关键词
Black henbane; Bubble bioreactors; Hairy roots; Hyoscyamus niger; Methyl jasmonate (elicitor); Scopolamine; Spray bioreactor; Tropane alkaloids; CULTURES;
D O I
10.1007/s10529-013-1426-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production. The latter gave the highest anisodamine content (0.67 mg/g dry wt) whereas scopolamine, hyoscyamine and cuscohygrine concentrations were highest in the bubble-column reactor (5.3, 1.6 and 26.5 mg/g dry wt, respectively). Both bioreactors gave similar productivities of scopolamine (1 and 0.98 mg/l day) and cuscohygrine (5 and 5.4 mg/l day), but anisodamine productivity was 3.5-fold higher in the hybrid bioreactor (HB) (0.02 and 0.07 mg/l day, respectively). Elicitation with methyl jasmonate increased scopolamine productivity by 146 % in roots grown in the HB whereas their permeabilization with DMSO caused 4-, 5-, 25- and 28-fold increase in scopolamine, hyoscyamine, anisodamine and cuscohygrine concentrations in the growth medium. In situ extraction with Amberlite XAD-2 doubled scopolamine productivity in the hybrid reactor after 50 days.
引用
收藏
页码:843 / 853
页数:11
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