Proteomic investigation of human cystic echinococcosis in the liver

被引：13

作者：

Longuespee, Remi ^{[1
,2
]}

Casadonte, Rita ^{[1
]}

Kriegsmann, Mark ^{[2
]}

Wandernoth, Petra ^{[3
]}

Lisenko, Katharina ^{[4
]}

Mazzucchelli, Gabriel ^{[5
]}

Becker, Michael ^{[6
]}

Kriegsmann, Joerg ^{[1
,3
,7
]}

机构：

[1] Proteopath GmbH, Max Planck Str 17, D-54296 Trier, Germany

[2] Heidelberg Univ, Inst Pathol, Neuenheimer Feld 224, D-69120 Heidelberg, Germany

[3] Mol Pathol Trier, Max Planck Str 17, D-54296 Trier, Germany

[4] Heidelberg Univ, Dept Internal Med 5, Neuenheimer Feld 410, D-69120 Heidelberg, Germany

[5] Univ Liege, GIGA Res, Syst Biol & Chem Biol, Mass Spectrometry Lab LSM, Allee 6 aoUt 11, B-4000 Liege, Belgium

[6] Bruker Daltonik GmbH, Fahrenheitstr, D-28359 Bremen, Germany

[7] MVZ Histol Cytol & Mol Diagnost Trier, Max Planck Str 5, D-54296 Trier, Germany

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2017年 / 211卷

关键词：

Histoproteomics; Cystic echinococcosis; Laser microdissection; Parasitic disease; MALDI IMS; PARAFFIN-EMBEDDED TISSUE; IMAGING MASS-SPECTROMETRY; GRANULOSUS METACESTODE; LARVAL; TOOL; INFECTION; REVEALS; CANCER;

D O I：

10.1016/j.molbiopara.2016.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cystic echinococcosis (CE) is a pandemic infectious disease caused by the tapeworm Echinococcus granulosus that forms cysts in different organs such as lungs and liver. Imaging examination and serological tests have some drawbacks such as low sensitivity. In this study, we used an up-to-date workflow of laser microdissection-based microproteomics and matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry in order to depict the proteomic pattern of CE in the liver. This investigation revealed specific markers of a parasitic cyst in liver. This proteomic pattern could facilitate diagnosis of CE in the future. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：9 / 14

页数：6

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