A correlation between altered O-GlcNAcylation, migration and with changes in E-cadherin levels in ovarian cancer cells

被引：42

作者：

Jin, Feng-zhen ^{[1
,2
]}

Yu, Chao ^{[2
]}

Zhao, De-zhang ^{[2
]}

Wu, Ming-jun ^{[2
]}

Yang, Zhu ^{[1
,2
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Chongqing 400010, Peoples R China

[2] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2013年 / 319卷 / 10期

关键词：

O-linked beta-N-acetylglucosamine transferase; O-GlcNAcylation; E-cadherin; Cell migration; Ovarian cancer; BETA-N-ACETYLGLUCOSAMINE; INSULIN-RESISTANCE; GLCNAC MODIFICATION; NUTRIENT SENSOR; C-MYC; GLYCOSYLATION; TRANSFERASE; PHOSPHORYLATION; ADHESION; CATENIN;

D O I：

10.1016/j.yexcr.2013.03.013

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

O-GlcNAcylation is a dynamic and reversible posttranslational modification of nuclear and cytoplasmic proteins. In recent years, the roles of O-GlcNAcylation in several human malignant tumors have been investigated, and O-GlcNAcylation was found to be linked to cellular features relevant to metastasis. In this study, we modeled four diverse ovarian cancer cells and investigated the effects of O-GlcNAcylation on ovarian cancer cell migration. We found that total O-GlcNAcylation level was elevated in HO-8910PM cells compared to OVCAR3 cells. Additionally, through altering the total O-GlcNAcylation level by OGT silencing or OGA inhibition, we found that the migration of OVCAR3 cells was dramatically enhanced by PUGNAc and Thiamet G treatment, and the migration ability of HO-8910PM cells was significantly inhibited by OGT silencing. Furthermore, we also found that the expression of E-cadherin, an O-GlcNAcylated protein in ovarian cancer cells, was reduced by OGA inhibition in OVCAR3 cells and elevated by OGT silencing in HO-8910PM cells. These results indicate that O-GlcNAcylation could enhance ovarian cancer cell migration and decrease the expression of E-cadherin. Our studies also suggest that O-GlcNAcylation might become another potential target for the therapy of ovarian cancer. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：1482 / 1490

页数：9

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