Mass spectrometry of macromolecular assemblies: preservation and dissociation

被引:167
作者
Benesch, JL [1 ]
Robinson, CV [1 ]
机构
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
基金
英国医学研究理事会;
关键词
D O I
10.1016/j.sbi.2006.03.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mass spectrometry not only plays a crucial role in the identification of proteins involved in the intricate interaction networks of the cell, but also is increasingly involved in the characterization of the non-covalent complexes formed by interacting partners. Recent developments have enabled the use of gas phase dissociation to probe oligomeric organization and topology, and increased understanding of the electrospray process is leading to knowledge of the structure of protein assemblies both in solution and in the gas phase.
引用
收藏
页码:245 / 251
页数:7
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