Effect of Nanoparticles Surface Charge on the Arabidopsis thaliana (L.) Roots Development and Their Movement into the Root Cells and Protoplasts

被引:48
作者
Milewska-Hendel, Anna [1 ]
Zubko, Maciej [2 ,3 ]
Stroz, Danuta [2 ]
Kurczynska, Ewa U. [1 ]
机构
[1] Univ Silesia Katowice, Fac Biol & Environm Protect, Dept Cell Biol, 28 Jagiellonska St, PL-40032 Katowice, Poland
[2] Univ Silesia Katowice, Inst Mat Sci, Fac Comp Sci & Mat Sci, 75 Pulku Piechoty St 1a, PL-41500 Chorzow, Poland
[3] Univ Hradec Kralove, Dept Phys, Hradec Kralove 50003, Czech Republic
关键词
Arabidopsis thaliana; gold nanoparticles; protoplasts; root development; root meristem; ultrastructure; COPPER-OXIDE NANOPARTICLES; ORYZA-SATIVA L; SILICON NANOPARTICLES; GOLD NANOPARTICLES; ZNO NANOPARTICLES; TROPHIC TRANSFER; MERISTEM GROWTH; QUANTUM DOTS; PORE-SIZE; PLANTS;
D O I
10.3390/ijms20071650
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Increasing usage of gold nanoparticles (AuNPs) in different industrial areas inevitably leads to their release into the environment. Thus, living organisms, including plants, may be exposed to a direct contact with nanoparticles (NPs). Despite the growing amount of research on this topic, our knowledge about NPs uptake by plants and their influence on different developmental processes is still insufficient. The first physical barrier for NPs penetration to the plant body is a cell wall which protects cytoplasm from external factors and environmental stresses. The absence of a cell wall may facilitate the internalization of various particles including NPs. Our studies have shown that AuNPs, independently of their surface charge, did not cross the cell wall of Arabidopsis thaliana (L.) roots. However, the research carried out with using light and transmission electron microscope revealed that AuNPs with different surface charge caused diverse changes in the root's histology and ultrastructure. Therefore, we verified whether this is only the wall which protects cells against particles penetration and for this purpose we used protoplasts culture. It has been shown that plasma membrane (PM) is not a barrier for positively charged (+) AuNPs and negatively charged (-) AuNPs, which passage to the cell.
引用
收藏
页数:22
相关论文
共 93 条
[1]   Enhanced mitotic division of cultured Passiflora and Petunia protoplasts by oxygenated perfluorocarbon and haemoglobin [J].
Anthony, P ;
Davey, MR ;
Power, JB ;
Lowe, KC .
BIOTECHNOLOGY TECHNIQUES, 1997, 11 (08) :581-584
[2]   Effects of silicon nanoparticles on molecular, chemical, structural and ultrastructural characteristics of oat (Avena sativa L.) [J].
Asgari, Faride ;
Majd, Ahmad ;
Jonoubi, Parissa ;
Najafi, Farzaneh .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2018, 127 :152-160
[3]   DISTRIBUTION AND REDISTRIBUTION OF EXTENSION GROWTH ALONG VERTICAL AND HORIZONTAL GRAVIREACTING MAIZE ROOTS [J].
BARLOW, PW ;
RATHFELDER, EL .
PLANTA, 1985, 165 (01) :134-141
[4]  
Benyettou F., 2012, Journal of Bioanalysis Biomedicine, V4, P39, DOI [DOI 10.4172/1948-593X.1000062, 10.4172/1948-593X.1000062 .]
[5]   2005 Critical Review: Nanoparticles and the environment [J].
Biswas, P ;
Wu, CY .
JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION, 2005, 55 (06) :708-746
[6]   Developmental and Reproductive Effects of Iron Oxide Nanoparticles in Arabidopsis thaliana [J].
Bombin, Sergey ;
LeFebvre, Mitchell ;
Sherwood, Jennifer ;
Xu, Yaolin ;
Bao, Yuping ;
Ramonell, Katrina M. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (10) :24174-24193
[7]   Ozone foliar symptoms in woody plant species assessed with ultrastructural and fluorescence analysis [J].
Bussotti, F ;
Agati, G ;
Desotgiu, R ;
Matteini, P ;
Tani, C .
NEW PHYTOLOGIST, 2005, 166 (03) :941-955
[8]   DETERMINATION OF THE PORE-SIZE OF CELL-WALLS OF LIVING PLANT-CELLS [J].
CARPITA, N ;
SABULARSE, D ;
MONTEZINOS, D ;
DELMER, DP .
SCIENCE, 1979, 205 (4411) :1144-1147
[9]   The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line [J].
Clift, Martin J. D. ;
Rothen-Rutishauser, Barbara ;
Brown, David M. ;
Duffin, Rodger ;
Donaldson, Ken ;
Proudfoot, Lorna ;
Guy, Keith ;
Stone, Vicki .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2008, 232 (03) :418-427
[10]   Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa [J].
Da Costa, M. V. J. ;
Sharma, P. K. .
PHOTOSYNTHETICA, 2016, 54 (01) :110-119