Evaluation of Stem Cells' Growth on Electrospun Polycaprolactone (PCL) Scaffolds Used for Soft Tissue Applications

被引:22
作者
Jabur, Akram R. [1 ]
Al-Hassani, Emad S. [1 ]
Al-Shammari, Ahmed M. [2 ]
Najim, Manar A. [1 ]
Hassan, Aymen A. [2 ]
Ahmed, Aesar A. [2 ]
机构
[1] Univ Technol Baghdad, Dept Mat Engn, Baghdad 10001, Iraq
[2] Univ Mustansiriyah, Iraqi Ctr Canc & Med Res, Baghdad 10001, Iraq
来源
INTERNATIONAL CONFERENCE ON TECHNOLOGIES AND MATERIALS FOR RENEWABLE ENERGY, ENVIRONMENT AND SUSTAINABILITY, TMREES17 | 2017年 / 119卷
关键词
Tissue Engineering; Soft tissue; Electrospinning; Biodegradable polymers; Stem cells; Scaffolds; BOVINE ARTICULAR CHONDROCYTES; EXTRACELLULAR-MATRIX; POLYMER SCAFFOLDS; FIBERS;
D O I
10.1016/j.egypro.2017.07.048
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study focuses on the preparation of a synthetic tissue from a biodegradable polymer that can be used for soft tissue applications using electrospinning technique. Biodegradable thermoplastic polymer which is polycaprolactone (PCL) used for making electrospun scaffolds in three concentrations (10, 15 and 20%) Weight/Volume PCL. The results revealed the increasing in fiber diameter with increasing PCL concentration in the prepared solutions for electrospinning. Then the growth of horse adipose derived mesenchymal stem cells was evaluated in vitro over these prepared scaffolds for 21 days with and without soaking in fetal bovine serum to study cells growth rate and proliferation over the scaffold surface. The Scanning electron microscope (SEM) analysis showed high growth rates for stem cells on these prepared scaffolds especially 15% w/v PCL which supported stem cells growth even when it was not soaked in serum. The histological analysis revealed that the suitable structure for cells spread and penetration resulted in 15 and 20% PCL scaffolds. In conclusions, these prepared scaffolds could be a candidate for use in soft tissues repair and thus for regenerative medicine application, because they highly supported stem cells growth. (C) 2017 The Authors. Published by Elsevier Ltd.
引用
收藏
页码:61 / 71
页数:11
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