Stochastic optical active rheology

被引：6

作者：

Lee, Hyungsuk ^{[3
]}

Shin, Yongdae ^{[4
]}

Kim, Sun Taek ^{[5
,6
,7
]}

Reinherz, Ellis L. ^{[5
,6
,7
]}

Lang, Matthew J. ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Dept Chem & Biomol Engn, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Mol Physiol & Biophys, Sch Med, Nashville, TN 37235 USA

[3] Yonsei Univ, Sch Mech Engn, Seoul 120749, South Korea

[4] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

[5] Dana Farber Canc Inst, Immunobiol Lab, Boston, MA 02115 USA

[6] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[7] Harvard Univ, Dept Med, Sch Med, Boston, MA 02115 USA

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 03期

基金：

美国国家科学基金会;

关键词：

NETWORKS; CELL; GELS;

D O I：

10.1063/1.4737159

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We demonstrate a stochastic based method for performing active rheology using optical tweezers. By monitoring the displacement of an embedded particle in response to stochastic optical forces, a rapid estimate of the frequency dependent shear moduli of a sample is achieved in the range of 10(-1)-10(3) Hz. We utilize the method to probe linear viscoelastic properties of hydrogels at varied cross-linker concentrations. Combined with fluorescence imaging, our method demonstrates non-linear changes of bond strength between T cell receptors and an antigenic peptide due to force-induced cell activation. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4737159]

引用

页数：4

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