Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum

被引：51
作者：
Varallyay, Eva [1 ]
Giczey, Gabor [1 ]
Burgyan, Jozsef [1 ]
机构：
[1] Agr Biotechnol Ctr, Plant Virol Grp, H-2100 Godollo, Hungary
来源：
ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 07期
关键词：
PLANT; BARLEY; WHEAT; DISCOVERY; PROTEIN; DEFENSE; FAMILY;
D O I：
10.1007/s00705-012-1286-y
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.
引用
收藏
页码：1345 / 1350
页数：6
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共 17 条
  • [1] Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function
    Bai, Yuling
    Pavan, Stefano
    Zheng, Zheng
    Zappel, Nana F.
    Reinstaedler, Anja
    Lotti, Concetta
    De Giovanni, Claudio
    Ricciardi, Luigi
    Lindhout, Pim
    Visser, Richard
    Theres, Klaus
    Panstruga, Ralph
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (01) : 30 - 39
  • [2] RNA silencing in plants
    Baulcombe, D
    [J]. NATURE, 2004, 431 (7006) : 356 - 363
  • [3] Widespread translational inhibition by plant miRNAs and siRNAs
    Brodersen, Peter
    Sakvarelidze-Achard, Lali
    Bruun-Rasmussen, Marianne
    Dunoyer, Patrice
    Yamamoto, Yoshiharu Y.
    Sieburth, Leslie
    Voinnet, Olivier
    [J]. SCIENCE, 2008, 320 (5880) : 1185 - 1190
  • [4] The barley mlo gene: A novel control element of plant pathogen resistance
    Buschges, R
    Hollricher, K
    Panstruga, R
    Simons, G
    Wolter, M
    Frijters, A
    vanDaelen, R
    vanderLee, T
    Diergaarde, P
    Groenendijk, J
    Topsch, S
    Vos, P
    Salamini, F
    Schulze-Lefert, P
    [J]. CELL, 1997, 88 (05) : 695 - 705
  • [5] Conserved requirement for a plant host cell protein in powdery mildew pathogenesis
    Consonni, Chiara
    Humphry, Matthew E.
    Hartmann, H. Andreas
    Livaja, Maren
    Durner, Jorg
    Westphal, Lore
    Vogel, John
    Lipka, Volker
    Kemmerling, Birgit
    Schulze-Lefert, Paul
    Somerville, Shauna C.
    Panstruga, Ralph
    [J]. NATURE GENETICS, 2006, 38 (06) : 716 - 720
  • [6] Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family
    Devoto, A
    Hartmann, HA
    Piffanelli, P
    Elliott, C
    Simmons, C
    Taramino, G
    Goh, CS
    Cohen, FE
    Emerson, BC
    Schulze-Lefert, P
    Panstruga, R
    [J]. JOURNAL OF MOLECULAR EVOLUTION, 2003, 56 (01) : 77 - 88
  • [7] Functional conservation of wheat and rice Mlo orthologs in defense modulation to the powdery mildew fungus
    Elliott, C
    Zhou, FS
    Spielmeyer, W
    Panstruga, R
    Schulze-Lefert, P
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2002, 15 (10) : 1069 - 1077
  • [8] Bacterially expressed dsRNA protects maize against SCMV infection
    Gan, Defang
    Zhang, Jiao
    Jiang, Haibo
    Jiang, Tong
    Zhu, Suwen
    Cheng, Beijiu
    [J]. PLANT CELL REPORTS, 2010, 29 (11) : 1261 - 1268
  • [9] Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley
    Hein, I
    Pacak, MB
    Hrubikova, K
    Williamson, S
    Dinesen, M
    Soenderby, IE
    Sundar, S
    Jarmolowski, A
    Shirasu, K
    Lacomme, C
    [J]. PLANT PHYSIOLOGY, 2005, 138 (04) : 2155 - 2164
  • [10] Barley stripe mosaic virus-induced gene silencing in a monocot plant
    Holzberg, S
    Brosio, P
    Gross, C
    Pogue, GP
    [J]. PLANT JOURNAL, 2002, 30 (03) : 315 - 327
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