Virus-induced gene silencing of Mlo genes induces powdery mildew resistance in Triticum aestivum

被引：52

作者：

Varallyay, Eva ^{[1
]}

Giczey, Gabor ^{[1
]}

Burgyan, Jozsef ^{[1
]}

机构：

[1] Agr Biotechnol Ctr, Plant Virol Grp, H-2100 Godollo, Hungary

来源：

ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 07期

关键词：

PLANT; BARLEY; WHEAT; DISCOVERY; PROTEIN; DEFENSE; FAMILY;

D O I：

10.1007/s00705-012-1286-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Powdery mildew is one of the most important cereal diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against this pathogen in barley. In this study, the possibility of inducing broad-spectrum powdery mildew resistance against this pathogen by RNAi of the barley Mlo ortholog in wheat was examined using virus-induced gene silencing (VIGS). A clear correlation was found between resistance and accumulation of Mlo-specific siRNAs, raising the possibility of designing powdery mildew resistance in wheat by RNA silencing using both transgenic and non-transgenic approaches.

引用

页码：1345 / 1350

页数：6

共 17 条

[1] Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function [J].

Bai, Yuling ;

Pavan, Stefano ;

Zheng, Zheng ;

Zappel, Nana F. ;

Reinstaedler, Anja ;

Lotti, Concetta ;

De Giovanni, Claudio ;

Ricciardi, Luigi ;

Lindhout, Pim ;

Visser, Richard ;

Theres, Klaus ;

Panstruga, Ralph .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (01) :30-39

[2] RNA silencing in plants [J].

Baulcombe, D .

NATURE, 2004, 431 (7006) :356-363

[3] Widespread translational inhibition by plant miRNAs and siRNAs [J].

Brodersen, Peter ;

Sakvarelidze-Achard, Lali ;

Bruun-Rasmussen, Marianne ;

Dunoyer, Patrice ;

Yamamoto, Yoshiharu Y. ;

Sieburth, Leslie ;

Voinnet, Olivier .

SCIENCE, 2008, 320 (5880) :1185-1190

[4] The barley mlo gene: A novel control element of plant pathogen resistance [J].

Buschges, R ;

Hollricher, K ;

Panstruga, R ;

Simons, G ;

Wolter, M ;

Frijters, A ;

vanDaelen, R ;

vanderLee, T ;

Diergaarde, P ;

Groenendijk, J ;

Topsch, S ;

Vos, P ;

Salamini, F ;

Schulze-Lefert, P .

CELL, 1997, 88 (05) :695-705

[5] Conserved requirement for a plant host cell protein in powdery mildew pathogenesis [J].

Consonni, Chiara ;

Humphry, Matthew E. ;

Hartmann, H. Andreas ;

Livaja, Maren ;

Durner, Jorg ;

Westphal, Lore ;

Vogel, John ;

Lipka, Volker ;

Kemmerling, Birgit ;

Schulze-Lefert, Paul ;

Somerville, Shauna C. ;

Panstruga, Ralph .

NATURE GENETICS, 2006, 38 (06) :716-720

[6] Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family [J].

Devoto, A ;

Hartmann, HA ;

Piffanelli, P ;

Elliott, C ;

Simmons, C ;

Taramino, G ;

Goh, CS ;

Cohen, FE ;

Emerson, BC ;

Schulze-Lefert, P ;

Panstruga, R .

JOURNAL OF MOLECULAR EVOLUTION, 2003, 56 (01) :77-88

[7] Functional conservation of wheat and rice Mlo orthologs in defense modulation to the powdery mildew fungus [J].

Elliott, C ;

Zhou, FS ;

Spielmeyer, W ;

Panstruga, R ;

Schulze-Lefert, P .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2002, 15 (10) :1069-1077

[8] Bacterially expressed dsRNA protects maize against SCMV infection [J].

Gan, Defang ;

Zhang, Jiao ;

Jiang, Haibo ;

Jiang, Tong ;

Zhu, Suwen ;

Cheng, Beijiu .

PLANT CELL REPORTS, 2010, 29 (11) :1261-1268

[9] Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley [J].

Hein, I ;

Pacak, MB ;

Hrubikova, K ;

Williamson, S ;

Dinesen, M ;

Soenderby, IE ;

Sundar, S ;

Jarmolowski, A ;

Shirasu, K ;

Lacomme, C .

PLANT PHYSIOLOGY, 2005, 138 (04) :2155-2164

[10] Barley stripe mosaic virus-induced gene silencing in a monocot plant [J].

Holzberg, S ;

Brosio, P ;

Gross, C ;

Pogue, GP .

PLANT JOURNAL, 2002, 30 (03) :315-327

← 1 2 →