Identification, phylogenetic analysis and characterization of obligate halophilic fungi isolated from a man-made solar saltern in Phetchaburi province, Thailand

被引:29
作者
Ali, Imran [1 ]
Kanhayuwa, Lakkhana [2 ]
Rachdawong, Sansanalak [2 ]
Rakshit, Sudip Kumar [1 ]
机构
[1] Asian Inst Technol, Sch Environm Resources & Dev, Klongluang 12120, Pathumthani, Thailand
[2] King Mongkuts Univ Technol Thonburi, Div Biotechnol, Sch Bioresources & Technol, Bangkok 10150, Thailand
关键词
Extremophiles; Halophilic fungi; Halotolerant fungi; Hypersaline environments; HYPERSALINE WATERS; ENVIRONMENTS; ADAPTATION; GROWTH;
D O I
10.1007/s13213-012-0540-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A total of 17 species from 43 isolates were obtained through serial dilutions of soil samples isolated from one of the man-made solar salterns located in Ban Laem district of Phetchaburi province, Thailand. Soil analysis of the sample revealed high salinity and moisture content, slight alkalinity and low amounts of nitrogen, total organic carbon and organic matter in the habitat. Morphological analysis was performed on all isolates, and molecular identification and phylogenetic analysis were carried out only on the halophilic fungi isolated. Six halophilic fungi, belonging to four species, were identified among the isolates, including five strains of Aspergillus genus [Aspergillus flavus, A. gracilis, A. penicillioides (2 strains) and A. restrictus]. One species was found to be a yeast, namely, Sterigmatomyces halophilus, which was the most frequent isolate found among the halophilic fungi. All other isolates were halotolerant fungi. Characterization of the halophilic fungal isolates showed that they were best adapted to conditions of 10-15 % NaCl (w/v), slight alkalinity (pH 7.0-7.5) and a temperature range of 30-35 A degrees C.
引用
收藏
页码:887 / 895
页数:9
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