MANIPULATION OF NI2+ TOLERANCE OF SACCHROMYCES CEREVISIAE CELLS: A PRIMARY STEP TO BIOREMEDIATION BY REMOVAL AND RECOVERY OF NI2+ FROM CONTAMINATED WATERS

被引：0

作者：

Farcasanu, Licana C. ^{[1
]}

Paraschivescu, Codruta ^{[1
]}

Ruta, Lavinia ^{[1
]}

Oprea, Eliza ^{[1
]}

Avramescu, Sorin ^{[1
]}

机构：

[1] Univ Bucharest, Dept Organ Chem, Fac Chem, Bucharest, Romania

来源：

REVUE ROUMAINE DE CHIMIE | 2008年 / 53卷 / 08期

关键词：

Saccharomyces cerevisiae; nickel; tolerance; hyperaccumulation;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Metal remediation through common physico-chemical techniques is expensive and unsuitable in case of voluminous effluents containing complex organic matter and low metal contamination. Alternative biotechnological approaches received great deal of attention in the recent years. Engineering cell lines that would hyperaccumulate heavy metals can be an invaluable tool in removing such ions from aqueous environments. In this study we obtained Saccharomyces cerevisiae mutants that were tolerant to high concentrations of Ni2+. Out of the five tolerant lines selected, two were shown to hyperaccumulate the Ni2+ ions in the vacuole. These mutants represent the first step in designing a system suitable for bioremediation of Ni2+-contaminated waters.

引用

页码：647 / +

页数：6

共 17 条

[1] Removal and recovery of nickel(II) from aqueous solution by loofa sponge-immobilized biomass of Chlorella sorokiniana:: characterization studies [J].

Akhtar, N ;

Iqbal, J ;

Iqbal, M .

JOURNAL OF HAZARDOUS MATERIALS, 2004, 108 (1-2) :85-94

[2] Microbial mats for multiple applications in aquaculture and bioremediation [J].

Bender, J ;

Phillips, P .

BIORESOURCE TECHNOLOGY, 2004, 94 (03) :229-238

[3]

BORSTPAUWELS GWFH, 1989, METHOD ENZYMOL, V174, P603

[4]

BOYLE RW, 1988, NICKEL ION ITS ROLE, V23, P123

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] 1-(2-PYRIDYLAZO)-2-NAPHTHOL AS A POSSIBLE ANALYTICAL REAGENT [J].

CHENG, KL ;

BRAY, RH .

ANALYTICAL CHEMISTRY, 1955, 27 (05) :782-785

[7] Role of L-histidine in conferring tolerance to Ni2+ in Sacchromyces cereviside cells [J].

Farcasanu, IC ;

Mizunuma, M ;

Nishiyama, F ;

Miyakawa, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2005, 69 (12) :2343-2348

[8] PROTEIN PHOSPHATASE 2B OF SACCHAROMYCES-CEREVISIAE IS REQUIRED FOR TOLERANCE TO MANGANESE, IN BLOCKING THE ENTRY OF IONS INTO THE CELLS [J].

FARCASANU, IC ;

HIRATA, D ;

TSUCHIYA, E ;

NISHIYAMA, F ;

MIYAKAWA, T .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 232 (03) :712-717

[9] The fate of Mn2+ ions inside Saccharomyces cerevisiae cells seen by electron paramagnetic resonance [J].

Farcasanu, IC ;

Ohta, N ;

Miyakawa, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (03) :468-471

[10] RNA-SYNTHESIS AND DEGRADATION DURING PREFERENTIAL INHIBITION OF PROTEIN-SYNTHESIS BY COBALT CHLORIDE IN ESCHERICHIA-COLI-K-12 [J].

GUHA, C ;

MOOKERJEE, A .

MOLECULAR BIOLOGY REPORTS, 1981, 7 (04) :217-220

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