Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis

被引:61
作者
Hong, Samin [1 ]
Kim, Chan Yun [1 ]
Lee, Jong Eun [2 ]
Seong, Gong Je [1 ]
机构
[1] Yonsei Univ, Coll Med, Dept Ophthalmol, Inst Vis Res, Seoul 135720, South Korea
[2] Yonsei Univ, Coll Med, Dept Anat, Seoul 135720, South Korea
关键词
Agmatine; Glaucoma; Retinal ganglion cell; Tumor necrosis factor; SPINAL-CORD-INJURY; OPTIC-NERVE HEAD; FACTOR-KAPPA-B; INTRAOCULAR-PRESSURE; TNF-ALPHA; GLIAL-CELLS; IN-VIVO; ISCHEMIA; GLAUCOMA; GLUTAMATE;
D O I
10.1016/j.lfs.2008.10.006
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aims: We investigated the protective effects of agmatine against tumor necrosis factor (TNF)-alpha-induced apoptosis in transformed rat retinal ganglion cells (RGC-5 cell line). Main methods: The RGC-5 cells were exposed to 50 ng/mL TNF-alpha for 48 h with or without presence of 100 W agmatine as indicated. Cell viability was determined by lactate dehydrogenase (LDH) assay. Double staining with Hoechst 33342 and propidium iodide for morphological analysis was performed. Subsequently, using annexin V assay, the proportion of cells actively undergoing apoptosis was determined. Key findings: After 48 h of exposure to 50 ng/mL TNF-alpha, 17.00% of RGC-5 cells were lost, as evident by LDH assay. TNF-alpha-induced RGC-5 cell death was reduced to 8.14% with 100 mu M agmatine treatment. This observed cell loss was due to apoptotic cell death, as established by annexin V assay. Significance: Our results reveal that agmatine has neuroprotective effects against TNF-alpha-induced apoptosis in retinal ganglion cells in vitro. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:28 / 32
页数:5
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