Identification of a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae

被引：69

作者：

Tokuoka, Masafumi ^{[1
]}

Seshime, Yasuyo ^{[2
]}

Fujii, Isao ^{[2
]}

Kitamoto, Katsuhiko ^{[3
]}

Takahashi, Tadashi ^{[1
]}

Koyama, Yasuji ^{[1
]}

机构：

[1] Noda Inst Sci Res, Chiba 2780037, Japan

[2] Iwate Med Univ, Sch Pharm, Lab Nat Prod Chem, Morioka, Iwate, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Microbiol Lab, Tokyo 1138654, Japan

来源：

FUNGAL GENETICS AND BIOLOGY | 2008年 / 45卷 / 12期

基金：

日本学术振兴会;

关键词：

Cyclopiazonic acid; Polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS); Secondary metabolite; Aspergillus oryzae;

D O I：

10.1016/j.fgb.2008.09.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cyclopiazonic acid (CPA) is a mycotoxin produced by several strains of Penicillium and Aspergillus species. Aspergillus oryzae strains used in fermented foods do not produce CPA; however, several wild-type A. oryzae strains produce CPA. Here, we identified a novel polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene involved in CPA production by comparing the telomere-adjacent region of a CPA-producing strain (A. oryzae NBRC 4177) with that of a nonproducing strain (A. oryzae R1B40). NBRC 4177 has an additional 17-18-kb sequence beyond the region corresponding to the telomere repeat in RIB40 and this additional regions contains 31 region of the PKS-NRPS gene, while RIB40 has only the 5' region of the PKS-NRPS gene. Gene disruption of the PKS-NRPS gene in NBRC 4177 resulted in elimination of CPA production. Thus, the PKS-NRPS gene is required for CPA biosynthesis, and the truncation of this gene is presumed as one of the determinants of CPA nonproductivity in A. oryzae RIB40. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：1608 / 1615

页数：8

共 17 条

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Prado-Alonso, Laura
Perez-Victoria, Ignacio
Malmierca, Monica G.
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Olano, Carlos
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2022, 88 (01)
[2] Functional expression of the Aspergillus flavus PKS-NRPS hybrid CpaA involved in the biosynthesis of cyclopiazonic acid
Seshime, Yasuyo
Juvvadi, Praveen Rao
Tokuoka, Masafumi
Koyama, Yasuji
Kitamoto, Katsuhiko
Ebizuka, Yutaka
Fujii, Isao
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[3] Nostophycin Biosynthesis Is Directed by a Hybrid Polyketide Synthase-Nonribosomal Peptide Synthetase in the Toxic Cyanobacterium Nostoc sp Strain 152
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[4] Biosynthesis of Long-Chain N-Acyl Amide by a Truncated Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrid Megasynthase in Fungi
Tang, Yi (yitang@ucla.edu), 1600, American Chemical Society (140):
[5] Biosynthesis of Long-Chain N-Acyl Amide by a Truncated Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrid Megasynthase in Fungi
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[10] Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis
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