Conductometric Biosensor Based on Flavocytochrome b2 for L-Lactate Determination

被引：4

作者：

Berezhetska, O. ^{[1
]}

Korpan, Y. ^{[1
]}

Gonchar, M. ^{[2
,3
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, UA-03680 Kiev 143, Ukraine

[2] Natl Acad Sci Ukraine, Inst Cell Biol, UA-79005 Lvov 5, Ukraine

[3] Rzeszow Univ, Fac Biotechnol, PL-36100 Kolbuszowa, Poland

来源：

SENSOR LETTERS | 2011年 / 9卷 / 06期

关键词：

Conductometric Biosensor; Hansenula Polymorpha; Flavocytochrome b(2); L-Lactate;

D O I：

10.1166/sl.2011.1796

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new conductometric biosensor based on flavocytochrome b(2) immobilized with ferricynide on gold planar microelectrodes, which serve as physical transducers, is presented. The developed biosensor is characterized by high selectivity to L-lactate. A number of working buffer solution parameters affecting the biosensor signal, i.e., pH, ion strength, and concentration, were studied. An influence of duration of bio-recognition molecule immobilization in glutaraldehyde vapor on the signal value was also investigated. The biosensor with optimized characteristics has sufficient stability and reproducibility and provides measurement of lactate concentration with the lower detection limit of 0.1 mM.

引用

页码：2388 / 2391

页数：4

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