Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation

被引：60

作者：

Ailion, Michael ^{[1
,2
]}

Hannemann, Mandy ^{[3
,4
]}

Dalton, Susan ^{[1
]}

Pappas, Andrea ^{[1
]}

Watanabe, Shigeki ^{[1
]}

Hegermann, Jan ^{[3
,5
]}

Liu, Qiang ^{[1
]}

Han, Hsiao-Fen ^{[1
]}

Gu, Mingyu ^{[1
]}

Goulding, Morgan Q. ^{[2
]}

Sasidharan, Nikhil ^{[3
]}

Schuske, Kim ^{[1
]}

Hullett, Patrick ^{[1
]}

Eimer, Stefan ^{[3
,5
]}

Jorgensen, Erik M. ^{[1
]}

机构：

[1] Univ Utah, Howard Hughes Med Inst, Dept Biol, Salt Lake City, UT 84112 USA

[2] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

[3] European Neurosci Inst, D-37077 Gottingen, Germany

[4] Int Max Planck Res Sch Mol Biol, D-37077 Gottingen, Germany

[5] DFG Res Ctr Mol Physiol Brain CMPB, D-37077 Gottingen, Germany

来源：

NEURON | 2014年 / 82卷 / 01期

关键词：

IMMATURE SECRETORY GRANULES; GTP-BINDING-PROTEINS; CAENORHABDITIS-ELEGANS; C-ELEGANS; RUN DOMAIN; RAB6-INTERACTING PROTEIN-1; HOMOTYPIC FUSION; TRANS-GOLGI; UNC-31; CAPS; PC12; CELLS;

D O I：

10.1016/j.neuron.2014.02.017

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network.

引用

页码：167 / 180

页数：14

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