Expression analysis of efflux pump genes among drug-susceptible and multidrug-resistant Mycobacterium tuberculosis clinical isolates and reference strains

被引：42

作者：

Calgin, Mustafa Kerem ^{[1
]}

Sahin, Fikret ^{[1
]}

Turegun, Buse ^{[1
]}

Gerceker, Devran ^{[1
]}

Atasever, Melike ^{[2
]}

Koksal, Deniz ^{[3
]}

Karasartova, Djursun ^{[1
]}

Kiyan, Mehmet ^{[1
]}

机构：

[1] Ankara Univ, Fac Med, Dept Microbiol, TR-06100 Ankara, Turkey

[2] MH Ataturk Chest Dis & Thorac Surg Training & Res, Dept Microbiol, TR-06290 Ankara, Turkey

[3] MH Ataturk Chest Dis & Thorac Surg Training & Res, Chest Dis Dept, TR-06290 Ankara, Turkey

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 76卷 / 03期

关键词：

Mycobacterium tuberculosis; Drug resistance; Efflux pumps; TRANSPORTERS; BOVIS;

D O I：

10.1016/j.diagmicrobio.2013.02.033

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Finding a gene or genes that are involved with multidrug resistance will be useful for finding a new target for the treatment of drug resistant tuberculosis. In this study, we aimed to compare the differences of the expression of 15 putative multidrug efflux pump genes in clinically isolated drug sensitive and multidrug resistant (MDR) Mycobacterium tuberculosis isolates, and reference strains. We found that these genes in the drug-sensitive and MDR M. tuberculosis isolates have similar rates of expressions. However, we found the expression levels of the all the genes are significantly higher in the clinical strains compared to the expression level of genes in the reference strains. In addition to this, it is found that standard strain has lower MIC value for the drugs including streptomycin and rifampin compared to the clinical isolate. We presume that the increase of the gene expression in the clinical strains is due to the exposure of antituberculosis drugs during treatment of patients, which cause constitutive expression of efflux systems, which might increase MIC levels of the major anti-tuberculosis drugs. (c) 2013 Elsevier Inc. All rights reserved.

引用

页码：291 / 297

页数：7

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