Construction of an improved RP4 (RK2)-based conjugative system

被引：31

作者：

Babic, Ana ^{[1
]}

Guerout, Anne-Marie ^{[1
]}

Mazel, Didier ^{[1
]}

机构：

[1] Inst Pasteur, Unite Plast Genome Bacterien, CNRS, URA 2171, F-75724 Paris, France

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 7-8期

关键词：

Conjugation; Suicide plasmids; Gene knock-out; RP4; plasmid; IncP alpha; Plasmid;

D O I：

10.1016/j.resmic.2008.06.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In many bacteria, gene replacement can only be achieved using RP4-based conjugative systems where exogenous DNA is delivered to the recipient on conditionally replicating plasmids carrying an oriT(RP4). In the commonly used strains SM10 and S17-1 conjugative functions are provided by an RP4 plasmid inserted in the chromosome. Those strains, besides mobilizing oriT(RP4)-carrying plasmids, also transfer their own chromosomal genes, which can be inconvenient for several reasons. We describe here a new version of in RP4-based conjugative system that allows for efficient transfer of mobilizable plasmids without the transfer of chromosomal genes, providing fully controlled and efficient exogeneous DNA delivery to non-transformable bacteria. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：545 / 549

页数：5

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