Structural characterization of α-galactosylated O-glycans from miniature pig kidney and endothelial cells

被引：5

作者：

Park, Hae-Min ^{[1
]}

Yang, Yung-Hun ^{[4
]}

Kim, Byung-Gee ^{[1
,2
,3
]}

Kim, Yun-Gon ^{[5
]}

机构：

[1] Seoul Natl Univ, Coll Engn, Sch Chem & Biol Engn, Seoul 151742, South Korea

[2] Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151742, South Korea

[3] Seoul Natl Univ, Inst Bioengn, Seoul 151742, South Korea

[4] Konkuk Univ, Coll Engn, Dept Microbial Engn, Seoul 143701, South Korea

[5] Soongsil Univ, Coll Engn, Dept Chem Engn, Seoul 156743, South Korea

来源：

CARBOHYDRATE RESEARCH | 2013年 / 369卷

基金：

新加坡国家研究基金会;

关键词：

O-Glycan; alpha-Galactosylated (alpha-Gal) glycan; Pig kidney; Pig endothelial cells; Mass spectrometric analysis; Xenotransplantation; MASS-SPECTROMETRY; GAL EPITOPE; CARBOHYDRATE; XENOTRANSPLANTATION; OLIGOSACCHARIDES; IDENTIFICATION; ANTIBODIES; GLYCOPROTEINS; ANTIGENS; TISSUES;

D O I：

10.1016/j.carres.2012.12.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

O-Glycan is a major oligosaccharide expressed on the surface of eukaryotic cells that plays a role in immune responses via cell-protein and cell-cell interaction. Unlike N-glycan and glycolipid-derived glycan, O-glycans have not been previously reported in pig organs. Here, we report the first instance of the identification of alpha 1,3 galactosylated O-glycans from pig kidney and endothelial cells by using MALDI-TOF MS and nano-ESI MS/MS. The MALDI profile showed 28 O-glycans from miniature pig kidney and 18 O-glycans from pig endothelial cells. Among them, three potential alpha-galactosylated glycans considered as immunogenic antigens were identified by CID using ESI-MS/MS; Hex alpha 1,3Hex-HexNAc-HexNAc, Hex alpha 1,3-Hex-Hex-HexNAc-HexNAc, and Hex alpha 1,3Hex-Hex-Hex-Hex-HexNAc. This structural information on glycans will provide significant information to clinical studies regarding immune rejection responses induced by carbohydrate antigens during xenotransplantation. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：48 / 53

页数：6

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