Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

被引：22

作者：

Feng, Peiying ^{[1
,2
]}

Klaassen, Corne H. W. ^{[3
]}

Meis, Jacques F. ^{[3
,4
]}

Najafzadeh, M. J. ^{[5
,6
,7
]}

Van den Ende, A. H. G. Gerrits ^{[2
]}

Xi, Liyan ^{[8
]}

de Hoog, G. S. ^{[2
,7
,8
,9
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou 510275, Guangdong, Peoples R China

[2] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands

[3] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands

[4] Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, NL-6525 ED Nijmegen, Netherlands

[5] Mashhad Univ Med Sci, Sch Med, Ghaem Hosp, Dept Parasitol & Mycol, Mashhad, Iran

[6] Mashhad Univ Med Sci, Sch Med, Ghaem Hosp, Canc Mol Pathol Res Ctr, Mashhad, Iran

[7] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands

[8] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China

[9] Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 03期

关键词：

RAPID IDENTIFICATION; EXOPHIALA-DERMATITIDIS; AROMATIC-HYDROCARBONS; PRACTICAL METHOD; ASPERGILLUS; CANDIDA; FUNGI; INFECTIONS; ORIGIN; SPP;

D O I：

10.1128/JCM.02898-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.

引用

页码：931 / 937

页数：7

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