Development of NIR Fluorescent Dyes Based on Si-rhodamine for in Vivo Imaging

被引：254
作者：
Koide, Yuichiro [1 ,3 ]
Urano, Yasuteru
Hanaoka, Kenjiro [1 ,3 ]
Piao, Wen [1 ,3 ]
Kusakabe, Moriaki [4 ]
Saito, Nae [2 ]
Terai, Takuya [1 ,3 ]
Okabe, Takayoshi [2 ]
Nagano, Tetsuo [1 ,3 ]
机构：
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Univ Tokyo, Open Innovat Ctr Drug Discovery, Bunkyo Ku, Tokyo 1130033, Japan
[3] Japan Sci & Technol Agcy, JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan
[4] Matrix Cell Res Inst Inc, Ushi Ku, Ibaraki 3001232, Japan
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 11期
关键词：
CANCER; MOUSE;
D O I：
10.1021/ja210375e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NW fluorescence probes.
引用
收藏
页码：5029 / 5031
页数：3
相关论文
共 20 条
  • [1] [Anonymous], 2005, INVITROGEN MOL PROBE
  • [2] Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands
    Becker, A
    Hessenius, C
    Licha, K
    Ebert, B
    Sukowski, U
    Semmler, W
    Wiedenmann, B
    Grötzinger, C
    [J]. NATURE BIOTECHNOLOGY, 2001, 19 (04) : 327 - 331
  • [3] Synthesis and evaluation of a novel fluorescent photoprobe for imaging matrix metalloproteinases
    Faust, Andreas
    Waschkau, Bianca
    Waldeck, Jens
    Hoeltke, Carsten
    Breyholz, Hans-Joerg
    Wagner, Stefan
    Kopka, Klaus
    Heindel, Walter
    Schaefers, Michael
    Bremer, Christoph
    [J]. BIOCONJUGATE CHEMISTRY, 2008, 19 (05) : 1001 - 1008
  • [4] In vivo near-infrared fluorescence imaging
    Frangioni, JV
    [J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (05) : 626 - 634
  • [5] A design concept of long-wavelength fluorescent analogs of rhodamine dyes: replacement of oxygen with silicon atom
    Fu, Meiyan
    Xiao, Yi
    Qian, Xuhong
    Zhao, Defeng
    Xu, Yufang
    [J]. CHEMICAL COMMUNICATIONS, 2008, (15) : 1780 - 1782
  • [6] In Vivo Fluorescence Imaging of E-Selectin Quantitative Detection of Endothelial Activation in a Mouse Model of Arthritis
    Gompels, Luke L.
    Madden, Leigh
    Lim, Ngee Han
    Inglis, Julia J.
    McConnell, Ellen
    Vincent, Tonia L.
    Haskard, Dorian O.
    Paleolog, Ewa M.
    [J]. ARTHRITIS AND RHEUMATISM, 2011, 63 (01): : 107 - 117
  • [7] Developments toward diagnostic breast cancer imaging using near-infrared optical measurements and fluorescent contrast agents
    Hawrysz, DJ
    Sevick-Muraca, EM
    [J]. NEOPLASIA, 2000, 2 (05): : 388 - 417
  • [8] Hicke BJ, 2006, J NUCL MED, V47, P668
  • [9] Development of an Si-Rhodamine-Based Far-Red to Near-Infrared Fluorescence Probe Selective for Hypochlorous Acid and Its Applications for Biological Imaging
    Koide, Yuichiro
    Urano, Yasuteru
    Hanaoka, Kenjiro
    Terai, Takuya
    Nagano, Tetsuo
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (15) : 5680 - 5682
  • [10] Evolution of Group 14 Rhodamines as Platforms for Near-Infrared Fluorescence Probes Utilizing Photoinduced Electron Transfer
    Koide, Yuichiro
    Urano, Yasuteru
    Hanaoka, Kenjiro
    Terai, Takuya
    Nagano, Tetsuo
    [J]. ACS CHEMICAL BIOLOGY, 2011, 6 (06) : 600 - 608
12