A Miniaturized Preconcentration Method Based on Dispersive Liquid-Liquid Microextraction for the Spectrophotometric Determination of Aziridine in Food Simulants

In this work a simple, rapid and sensitive method using dispersive liquid-liquid microextraction (DLLME) combined with UV-Vis spectrophotometry has been developed for the preconcentration and determination of trace amounts of aziridine in food simulants. The method is based on derivatization of aziridine with Folin's reagent (1,2-naphthoquione-4-sulphonic acid) and extraction of color product using DLLME technique. Some important parameters, such as reaction conditions, and type and volume of extraction solvent and disperser solvent were studied and optimized. Under optimum conditions, a linear calibration curve in the range of 2.0-350 ng mL(-1) of aziridine was obtained. Detection limit based on 3S(b) was 1.0 ng mL(-1), and the relative standard deviation for 50 ng mL(-1) of aziridine was 2.49c (n = 7). The proposed method was applied for the determination of aziridine in food simulants.