Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR

被引：15
作者：
Gruas, Cristina [1 ]
Llambi, Silvia [2 ]
Victoria Arruga, M. [1 ]
机构：
[1] Univ Zaragoza, Fac Vet, Lab Cytogenet & Mol Genet, E-50013 Zaragoza, Spain
[2] UDELAR, Fac Vet, Inst Prod Anim, Dept Genet & Mejora Anim, Montevideo 11600, Uruguay
来源：
ARCHIVES OF MICROBIOLOGY | 2014年 / 196卷 / 01期
关键词：
Legionella culture method; Real-time PCR; Legionella spp; Legionella pneumophila; POLYMERASE-CHAIN-REACTION; GENE PROBE METHODS; ENVIRONMENTAL WATER; IMMUNOFLUORESCENCE ASSAY; CONVENTIONAL CULTURE; LIGHTCYCLER PCR; MIP GENE; AMPLIFICATION; SCANVIT-LEGIONELLA(TM); IDENTIFICATION;
D O I：
10.1007/s00203-013-0934-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127-128, 2002; Doleans et al. in J Clin Microbiol 42:458-460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459-466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.
引用
收藏
页码：63 / 71
页数：9
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