The molecular mechanism regulating the autonomous circadian expression of Topoisomerase I in NIH3T3 cells

被引：9

作者：

Yang, Fang ^{[1
,2
]}

Nakajima, Yoshihiro ^{[3
]}

Kumagai, Megumi ^{[1
,2
]}

Ohmiya, Yoshihiro ^{[3
]}

Ikeda, Masaaki ^{[1
,2
]}

机构：

[1] Saitama Med Univ, Dept Physiol, Moroyama, Saitama 3500495, Japan

[2] Saitama Med Univ, Res Ctr Genom Med, Project Res Div, Mol Clock Project, Hidaka, Saitama 3501241, Japan

[3] AIST, Natl Inst Adv Ind Sci & Technol, Res Inst Cell Engn, Cell Dynam Res Grp, Osaka 5638577, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 380卷 / 01期

关键词：

Topoisomerase I; Circadian rhythms; Promoter analysis; Antitumor drugs; Clock genes; GENE-EXPRESSION; CLOCK; IDENTIFICATION; PROTEIN; RHYTHM; BMAL1; MOUSE;

D O I：

10.1016/j.bbrc.2008.12.186

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify whether Topoisomerase I (TopoI) has autonomous circadian rhythms regulated by clock genes, we tested mouse TopoI (mTopoI) promoter oscillation in NIH3T3 cells using a real-time monitoring assay and Topol mRNA oscillations using real-time RT-PCR. Analysis of the mTopol promoter region with MatInspector software revealed two putative E-box (E1 and E2) and one DBP/E4BP4-binding element (D-box). Luciferase assays indicated that mTopoI gene expression was directly regulated by clock genes. The real-time monitoring assay showed that E-box and D-box response elements participate in the regulation of the circadian expression of mTopoI. Furthermore, a gel-shift assay showed that E2 is a direct target of the BMAL1/CLOCK heterodimer and DBP binds to the Putative D-site. These results indicate that TopoI is expressed in an autonomous circadian rhythm in NIH3T3 cells. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：22 / 27

页数：6

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