Cellular microRNA miR-181b Inhibits Replication of Mink Enteritis Virus by Repression of Non-Structural Protein 1 Translation

被引：12

作者：

Sun, Jia-zeng ^{[1
]}

Wang, Jigui ^{[1
]}

Yuan, Daoli ^{[1
]}

Wang, Shuang ^{[1
]}

Li, Zhili ^{[1
]}

Yi, Bao ^{[1
]}

Mao, Yaping ^{[1
]}

Hou, Qiang ^{[1
]}

Liu, Weiquan ^{[1
]}

机构：

[1] China Agr Univ, State Key Lab Agrobiotechnol, Dept Biochem & Mol Biol, Coll Biol Sci, Beijing 100094, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

FELINE PANLEUKOPENIA VIRUS; CANINE HOST-RANGE; RNA INTERFERENCE; MINUTE VIRUS; PARVOVIRUS; EXPRESSION; GENE; SEQUENCE; MICE; PROTECTION;

D O I：

10.1371/journal.pone.0081515

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mink enteritis virus (MEV) is one of the most important viral pathogens in the mink industry. Recent studies have showed that microRNAs (miRNAs), small noncoding RNAs of length ranging from 18-23 nucleotides (nt) participate in host-pathogen interaction networks; however, whether or not miRNAs are involved in MEV infection has not been reported. Our study revealed that miRNA miR-181b inhibited replication of MEV in the feline kidney (F81) cell line by targeting the MEV non-structural protein 1 (NS1) messenger RNA (mRNA) coding region, resulting in NS1 translational repression, while MEV infection reduced miR-181b expression. This is the first description of cellular miRNAs modulating MEV infection in F81 cells, providing further insight into the mechanisms of viral infection, and may be useful in development of naturally-occurring miRNAs antiviral strategies.

引用

页数：14

共 69 条

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