Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002

被引：3

作者：

Lu, Xiaoyun ^{[1
,2
]}

Yi, Qiufen ^{[1
,2
]}

Zhang, Guofang ^{[1
,2
]}

Zhu, Xianming ^{[2
]}

Zhou, Honggang ^{[3
]}

Dong, Hui ^{[2
]}

机构：

[1] Tianjin Univ Sci & Technol, Tianjin 300222, Peoples R China

[2] Tianjin Int Joint Acad Biotechnol & Med, Tianjin 300457, Peoples R China

[3] Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷

基金：

中国国家自然科学基金;

关键词：

MYCOBACTERIUM-TUBERCULOSIS; SUBTILIS; CRYSTALS; MODEL;

D O I：

10.1107/S1744309113019672

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Alanine dehydrogenase (L-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD(+)-dependent interconversion of L-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His(6) tag by Ni2+-chelating affinity chromatography for X-ray crystallographic analysis. Crystals were grown in a solution consisting of 0.1 M HEPES pH 8.0, 12%(w/v) polyethylene glycol 8000, 8%(v/v) ethylene glycol at a concentration of 15 mg ml(-1) purified protein. The crystal diffracted to 2.35 angstrom resolution and belonged to the trigonal space group R32, with unit-cell parameters a = b = 125.918, c = 144.698 angstrom.

引用

页码：934 / 936

页数：3

共 50 条

[31] Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius
Muranova, TA
Ruzheinikov, SN
Sedelnikova, SE
Baker, PJ
Pasquo, A
Galkin, A
Esaki, N
Ohshima, T
Soda, K
Rice, DW
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 1059 - 1062
[32] Overexpression, crystallization and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Archaeoglobus fulgidus
Lee, Hyung Ho
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1556 - 1558
[33] Crystallization and preliminary X-ray analysis of geraniol dehydrogenase from Backhousia citriodora (lemon myrtle)
Saito, Yosuke
Ito, Sohei
Koltunow, Anna M.
Sakai, Hiroshi
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 665 - 667
[34] Crystallization and preliminary X-ray analysis of β-amylase from Bacillus cereus var. Mycoides
Oyama, T
Kusunoki, M
Kishimoto, Y
Takasaki, Y
Nitta, Y
PROTEIN AND PEPTIDE LETTERS, 1998, 5 (06): : 349 - 354
[35] Crystallization and preliminary X-ray analysis of phosphoserine aminotransferase from Bacillus circulans subsp alkalophilus
Moser, M
Muller, R
Battchikova, N
Koivulehto, M
Korpela, T
Jansonius, JN
PROTEIN SCIENCE, 1996, 5 (07) : 1426 - 1428
[36] Crystallization and preliminary X-ray diffraction analysis of monkey dimeric dihydrodiol dehydrogenase
El-Kabbani, O
Chung, RPT
Ramsland, PA
Asada, Y
Ishikura, S
Usami, N
Hara, A
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 163 - 164
[37] Crystallization and preliminary X-ray analysis of D-arabinose isomerase from Bacillus pallidus
Takeda, Kosei
Yoshida, Hiromi
Takada, Goro
Izumori, Ken
Kamitori, Shigehiro
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2008, 64 : C266 - C267
[38] Crystallization and preliminary X-ray analysis of the 6-phospho-α-glucosidase from Bacillus subtilis
Varrot, A
Yamamoto, H
Sekiguchi, J
Thompson, J
Davies, GJ
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 1212 - 1214
[39] Crystallization and preliminary X-ray diffraction analysis of the arginine repressor ArgR from Bacillus halodurans
Kang, Jina
Park, Young Woo
Yeo, Hyun Ku
Lee, Jae Young
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 : 291 - 294
[40] Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis
Ju, Hansol
Pandian, Ramesh
Kim, Kyungmin
Kim, Kyeong Kyu
Kim, T. Doohun
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 : 473 - 475

← 1 2 3 4 5 →