Fluorescence ratiometric assay for discriminating GSH and Cys based on the composites of UiO-66-NH2 and Cu nanoclusters

被引：45

作者：

Ma, Jinzhu ^{[1
]}

Lu, Zhangdi ^{[2
,3
]}

Li, Chenmin ^{[1
]}

Luo, Yingjian ^{[5
]}

Shi, Yu-e ^{[1
]}

Alam, Parvej ^{[2
,3
]}

Lam, Jacky W. Y. ^{[2
,3
]}

Wang, Zhenguang ^{[1
]}

Tang, Ben Zhong ^{[1
,2
,3
,4
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Chem Biol Hebei Prov, Key Lab Med Chem & Mol Diag,Minist Educ, Baoding 071002, Peoples R China

[2] Hong Kong Univ Sci & Technol, Guangdong Hong Kong Macao Joint Lab Optoelect & Ma, Dept Chem, Kowloon, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Guangdong Hong Kong Macao Joint Lab Optoelect & Ma, Kowloon, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen 518172, Guangdong, Peoples R China

[5] Hong Kong Univ Sci & Technol, Div Environm & Sustainabil, Kowloon, Hong Kong, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2022年 / 215卷

基金：

中国国家自然科学基金;

关键词：

Glutathione; Cysteine; Fluorescence ratiometric assay; Metal -organic frameworks; Cu nanoclusters; Aggregation -enhanced emission; SELECTIVE DETECTION; GLUTATHIONE; CYSTEINE; LUMINESCENCE; PROBES;

D O I：

10.1016/j.bios.2022.114582

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The discriminative detection of glutathione (GSH) from cysteine (Cys) remains a challenge because of their similarity in structure and chemical properties. This study reported a strategy for selective and sensitive detection of GSH based on the GSH-promoted blue fluorescence of UiO-66-NH2 and aggregation-enhanced emission (AEE) feature of orange emissive Cu nanoclusters (NCs). A relatively weak blue fluorescence of UiO-66-NH2 was converted to strong after reacting with GSH due to the rotation-restricted emission enhancement mechanism. In addition, the GSH-activated UiO-66-NH2 was further used as a template and reducing reagent for synthesizing orange-red AEE active Cu NCs composites (UiO-66-NH2@Cu NCs). A ratiometric fluorescence response was observed after forming UiO-66-NH2@Cu NCs, helping discriminate GSH over Cys. In addition, UiO-66-NH2@Cu NCs were further utilized for the detection of GSH in clinical samples. The present findings provide an efficient strategy to discriminate GSH over Cys and open a new door for utilizing and functionalizing metal -organic frameworks (MOFs) for various applications.

引用

页数：5

共 37 条

[1] Highly fluorescent copper nanoclusters for sensing and bioimaging [J].