Improved method for fatty acid analysis in herbage based on direct transesterification followed by solid-phase extraction

被引：41
|
作者：
Alves, Susana P. [1 ,2 ]
Cabrita, Ana R. J. [3 ]
Fonseca, Antonio J. M. [2 ]
Bessa, Rui J. B. [1 ]
机构：
[1] Inst Nacl Recursos Biol, Unidade Prod Anim, REQUIMTE, P-2005048 Fonte Boa, Vale Santarem, Portugal
[2] Univ Porto, Inst Ciencias Biomed Abel Salazar, ICBAS, REQUIMTE, P-4485661 Vairao Vc, Portugal
[3] Univ Porto, Fac Ciencias, SAECA, REQUIMTE, P-4485661 Vairao Vc, Portugal
来源：
JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1209卷 / 1-2期
关键词：
Fatty acid methyl esters; Gas-liquid chromatography; Forage plants;
D O I：
10.1016/j.chroma.2008.08.114
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Direct transesterification (DT) and solvent extraction with acid or basic derivatization procedure for fatty acid (FA) analysis in herbage were compared. The highest total FA, alpha-linolenic and linoleic acid contents were obtained with DT. However, DT also produced the highest amount of interfering compounds, identified as phytadienes and sugar derivative products, which may co-elute with FA. An additional step based on solid-phase extraction to produce clean samples was proposed. This procedure is simple and gives good recoveries for the FA fortified samples. Additionally, structural characterization of 16:1 trans-3 was conducted by covalent adduct chemical ionization tandem mass spectrometry. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：212 / 219
页数：8
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