A Two-photon Fluorescent Probe for Intracellular Detection of Tyrosinase Activity

被引：45

作者：

Yan, Shengyong ^{[1
]}

Huang, Rong ^{[1
]}

Wang, Changcheng ^{[1
]}

Zhou, Yimin ^{[1
]}

Wang, Jiaqi ^{[1
]}

Fu, Boshi ^{[1
]}

Weng, Xiaocheng ^{[1
]}

Zhou, Xiang ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Biomed Polymers,State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China

[2] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2012年 / 7卷 / 12期

基金：

美国国家科学基金会;

关键词：

cell imaging; enzymes; fluorescent probes; two-photon; tyrosinase; MELANOMA-CELLS; PRODRUGS; CANDIDATES; OXIDATION; DOPAMINE;

D O I：

10.1002/asia.201200762

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AAN effective sensor: The two-photon turn-on fluorescent probe NHU was synthesized to optically detect tyrosinase activity in vitro and in melanoma cells. NHU is composed of a 4-aminophenol moiety and a naphthylamine unit, both of which are connected through a urea linkage. Upon exposure to tyrosinase, the 4-aminophenol site is gradually oxidized to the corresponding orthoquinone, ultimately releasing the highly fluorescent product 6-acyl-N-methyl-2- naphthylamine (AAN). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2782 / 2785

页数：4

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