Two new dicopper(II) complexes with oxamido-bridged ligand: Synthesis, crystal structures, DNA binding/cleavage and BSA binding activity

New oxamido-bridged copper(II) complexes, [Cu-2(oxbP)(H2O)(2)(NCS)(2)] (1) and [Cu-2(oxbp)(mu-DMSO)(2)(NCS)(2)] [Cu-2(oxbp)(DMSO)(2)(NCS)(2)] (2) (H(2)oxbp = N,N'-bis(2-(diethylamino)ethyl)oxalamide), were synthesized and characterized by single-crystal X-ray diffraction, elemental analysis, IR, and electronic spectra. X-ray analysis revealed that complex 1 consists of neutral binuclear [Cu-2(oxbP)(H2O)(2)(NCS)(2)] units which forms a two-dimensional network through intermolecular hydrogen bonds and complex 2 is constructed by neutral [Cu-2(oxbp)(mu-DMSO)(2)(NCS)(2)] (2a) and [Cu-2(oxbp)(DMSO)(2)(NCS)(2)] (2b) entities which alternately distribute to form a two-dimensional network by means of quasi p-DMSO bridge and intermolecular hydrogen bonds. In both 1 and 2, bicopper centers are linked by the "trans-form" oxamido bridges with the distances of 5272 angstrom for 1 and ay. 5.296 angstrom for 2, respectively. The interaction of Cu(II) complexes with DNA was investigated by UV-visible, fluorescence emission spectrometry and agarose gel electrophoresis. The apparent binding constant (K-app) values of 3.16 x 10(5) M-1 for 1 and 4.9 x 10(5) M-1 for 2 suggest moderate intercalative binding modes between the complexes and DNA. Complex 1 displayed efficient oxidative cleavage of supercoiled DNA, which might indicate that the underlying mechanism involves singlet oxygen (O-1(2)) as reactive oxygen species. Complex 2 is characteristic of the involvement of a singlet oxygen-like entity and hydrogen peroxide in the cleavage process. In addition, our present work showed, by fluorescence spectrometry of BSA with complexes, both 1 and 2 bind to BSA with a medium affinity through a static mode which is tentatively assigned binding to Trp134 in BSA. (C) 2012 Elsevier Inc. All rights reserved.