Piecemeal microautophagy of the nucleus Genetic and morphological traits

被引：39

作者：

Krick, Roswitha ^{[1
]}

Muhe, Yvonne ^{[1
]}

Prick, Tanja ^{[1
]}

Bredschneider, Monika ^{[1
]}

Bremer, Sebastian ^{[1
]}

Wenzel, Dirk ^{[3
]}

Eskelinen, Eeva-Liisa ^{[2
]}

Thumm, Michael ^{[1
]}

机构：

[1] Univ Gottingen, Ctr Biochem & Mol Cell Biol, D-37073 Gottingen, Lower Saxony, Germany

[2] Univ Helsinki, Dept Biol & Environm Sci, Helsinki, Finland

[3] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

来源：

AUTOPHAGY | 2009年 / 5卷 / 02期

关键词：

piecemeal microautophay of the nucleus; micronucleophagy; OXYSTEROL-BINDING PROTEIN; VACUOLE JUNCTIONS; AUTOPHAGOSOME FORMATION; YEAST; MACHINERY; PEXOPHAGY; HOMOLOG; TSC13P; OSH1P;

D O I：

10.4161/auto.5.2.7639

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.

引用

页码：270 / 272

页数：3

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