Piecemeal microautophagy of the nucleus Genetic and morphological traits

被引：38

作者：

Krick, Roswitha ^{[1
]}

Muhe, Yvonne ^{[1
]}

Prick, Tanja ^{[1
]}

Bredschneider, Monika ^{[1
]}

Bremer, Sebastian ^{[1
]}

Wenzel, Dirk ^{[3
]}

Eskelinen, Eeva-Liisa ^{[2
]}

Thumm, Michael ^{[1
]}

机构：

[1] Univ Gottingen, Ctr Biochem & Mol Cell Biol, D-37073 Gottingen, Lower Saxony, Germany

[2] Univ Helsinki, Dept Biol & Environm Sci, Helsinki, Finland

[3] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

来源：

AUTOPHAGY | 2009年 / 5卷 / 02期

关键词：

piecemeal microautophay of the nucleus; micronucleophagy; OXYSTEROL-BINDING PROTEIN; VACUOLE JUNCTIONS; AUTOPHAGOSOME FORMATION; YEAST; MACHINERY; PEXOPHAGY; HOMOLOG; TSC13P; OSH1P;

D O I：

10.4161/auto.5.2.7639

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.

引用

页码：270 / 272

页数：3

共 19 条

[1] Dunn WA, 2005, AUTOPHAGY, V1, P75
[2] Peroxisome turnover by micropexophagy:: an autophagy-related process
Farré, JC
Subramani, S
[J]. TRENDS IN CELL BIOLOGY, 2004, 14 (09) : 515 - 523
[3] Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae
Kohlwein, SD
Eder, S
Oh, CS
Martin, CE
Gable, K
Bacikova, D
Dunn, T
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (01) : 109 - 125
[4] Piecemeal Microautophagy of the Nucleus Requires the Core Macroautophagy Genes
Krick, R.
Muehe, Y.
Prick, T.
Bremer, S.
Schlotterhose, P.
Eskelinen, E-L.
Millen, J.
Goldfarb, D. S.
Thumm, M.
[J]. MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (10) : 4492 - 4505
[5] Dissecting the localization and function of Atg18, Atg21 and Ygr223c
Krick, Roswitha
Henke, Sandra
Tolstrup, Joern
Thumm, Michael
[J]. AUTOPHAGY, 2008, 4 (07) : 896 - 910
[6] Targeting of Tsc13p to nucleus-vacuole junctions: A role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles
Kvam, E
Gable, K
Dunn, TM
Goldfarb, DS
[J]. MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (09) : 3987 - 3998
[7] Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae
Kvam, E
Goldfarb, DS
[J]. JOURNAL OF CELL SCIENCE, 2004, 117 (21) : 4959 - 4968
[8] Nucleus-vacuole junctions and piecemeal microautophagy of the nucleus in S-cerevisiae
Kvam, Erik
Goldfarb, David S.
[J]. AUTOPHAGY, 2007, 3 (02) : 85 - 92
[9] Autophagy and the cvt pathway both depend on AUT9
Lang, T
Reiche, S
Straub, M
Bredschneider, M
Thumm, M
[J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (08) : 2125 - 2133
[10] Autophagy in the pathogenesis of disease
Levine, Beth
Kroemer, Guido
[J]. CELL, 2008, 132 (01) : 27 - 42

← 1 2 →