Optimization of Multi-enzyme Production by Fungi Isolated from Palm Kernel Expeller using Response Surface Methodology

被引：0

作者：

Chen, Wei Li ^{[1
]}

Liang, Juan Boo ^{[1
]}

Jahromi, Mohammed Faseleh ^{[1
]}

Ho, Yin Wan ^{[2
]}

Abdullah, Norhani ^{[1
,3
]}

机构：

[1] Univ Putra Malaysia, Inst Trop Agr, Upm Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Inst Biosci, Upm Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Biochem, Upm Serdang 43400, Selangor, Malaysia

来源：

BIORESOURCES | 2013年 / 8卷 / 03期

关键词：

Solid-state fermentation; Aspergillus terreus; Palm kernel expeller; Response surface methodology; SOLID-STATE FERMENTATION; XYLANASE PRODUCTION; ASPERGILLUS-AWAMORI; ENZYME-PRODUCTION; BETA-MANNANASE; PURIFICATION; TEMPERATURE; SUBSTRATE; NIGER; MEAL;

D O I：

暂无

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

Response surface methodology (RSM) was used to optimize the co-production of a mixture of crude cellulosic and hemicellulosic enzymes (endoglucanase, xylanase, and mannanase) by Aspergillus terreus K1 in solid-state fermentation (SSF) using palm kernel expeller (PKE) as the sole carbon source. These enzymes have gained renewed interest due to their efficacy to improve the digestibility of PKE for use in diets of mono-gastric animals (poultry, pigs, and fish). The results showed that temperature, moisture, inoculum concentration, and initial pH had significant (P< 0.05) effects on the enzymes production. Using PKE as a solid substrate, maximum endoglucanase, mannanase, and xylanase (17.37, 41.24, and 265.57 U/g DM, respectively) were obtained at 30.5 degrees C, 62.7% moisture, 6% inoculum, and pH 5.8. The enzyme activities recorded were close to the predicted values (19.97, 44.12, and 262.01 U/g DM, respectively).

引用

页码：3844 / 3857

页数：14

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