Angiotensin-(1-7) enhances angiotensin II induced phosphorylation of ERK1/2 In mouse bone marrow-derived dendritic cells

It is well known that angiotensin-(1-7)(Ang-(1-7)) counterbalances vasoconstrictive and proliferative functions of angiotensin II (Ang II). some of those actions are via inhibition of Ang II induced activation of mitogen-activated protein kinases(MAPK). This study investigated the effects of Ang-(1-7) on Ang II-mediated cell signaling pathways in mouse bone marrow-derived dendritic cells (DC). The expression of receptor Mas and angiotensin-converting enzyme-related carboxypeptidase (ACE2) mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR); activation of MAPK was detected by immunoblotting after incubation of dendritic cells with Ang II in the presence or absence of Ang-(1-7), valsartan, PD123319, and D-Ala(7)-Ang-(1-7). Ang II rapidly (5min, 10(-7) mol/L) stimulated phosphorylation of extracellular signal-related kinase (ERK1/2): this effect was partially inhibited by Ang II type 1 (AT1) receptor antagonist valsartan and Significantly attenuated by Ang II type 2 (AT2) receptor antagonist PD123319. Ang-(1-7) alone also induced phosphorylation of ERK1/2: co-treatment of Ang-(1-7) and Ang II markedly enhanced ERK1/2 phosphorylation, the enhancement was eliminated by the Ang(1-7) receptor antagonist D-Ala(7)-Ang-(1-7). Both Ang-(1-7) and Ang II had no effect on p38 and c-Jun N-terminal kinase (JNK) phosphorylation. In conclusion, Ang II stimulates ERK1/2 phosphorylation via AT2 receptor in mouse DC, Ang-(1-7) enhances this effect. Generation of Ang-(1-7) by DC could thereby Counteract on the pro-inflammatory function of locally generated Ang II. (C) 2008 Elsevier Ltd. All rights reserved.