Investigating Protein-Protein Interactions by Far-Westerns

被引：7

作者：

Chan, Catherine S. ^{[1
]}

Winstone, Tara A. L. ^{[1
]}

Turner, Raymond J. ^{[1
]}

机构：

[1] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada

来源：

PROTEIN - PROTEIN INTERACTION | 2008年 / 110卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Blot overlay; Far-Western; Protein-protein interactions; Sandwich ELISA; Sandwich Western;

D O I：

10.1007/10_2007_090

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The identification of protein interaction partners can often elucidate the function of the protein under investigation based on the "guilty by association" concept. Furthermore, the binding event between two proteins can be used as a functional assay when no such assay is avadable. Despite the large number of advanced techniques that are currently available for studying protein-protein interactions, far-Westerns or blot overlays are still very commonly used in the average laboratory setting due to their powerfulness. This is due to the simplicity and clarity in the results that they produce. Here, the details and mechanics of far-Westerns are discussed to help the reader choose amongst the different variations that exist depending on the question being investigated and the materials available to them. Some examples involving unique questions are also discussed in order to educate the reader on the versatility of far-Westerns. Finally, a troubleshooting section provides the reader with an understanding of the common problems that can be encountered and how these problems can be circumvented.

引用

页码：195 / 214

页数：20

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