The ascorbate peroxidase 1 regulates ascorbic acid metabolism in fresh-cut leaves of tea plant during postharvest storage under light/dark conditions

被引:16
作者
Li, Hui [1 ]
Liu, Jie-Xia [2 ]
Wang, Yu [1 ]
Zhuang, Jing [1 ]
机构
[1] Nanjing Agr Univ, Coll Hort, Tea Sci Res Inst, Minist Agr & Rural Affairs,Key Lab Biol & Germpla, Nanjing 210095, Peoples R China
[2] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
基金
中国国家自然科学基金;
关键词
Ascorbic acid; Ascorbate peroxidase; Postharvest storage; Subcellular localization; Arabidopsis; Tea plant; GENE-EXPRESSION; VITAMIN-C; QUALITY; SPINACH; ACCUMULATION; TEMPERATURE; GLUTATHIONE; ATMOSPHERE; ISOENZYMES; EXPOSURE;
D O I
10.1016/j.plantsci.2020.110500
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Postharvest storage conditions affect the ascorbic acid (AsA) levels in fresh-cut leaves of horticultural crops. However, the detailed mechanism of AsA metabolism in the fresh-cut leaves of tea plant (Camellia sinensis) during postharvest storage under light/dark conditions remains unclear. To investigate the AsA mechanism, we treated fresh-cut tea leaves with light/dark during postharvest storage. An ascorbate peroxidase 1 (CsAPX1) protein involved in AsA metabolism was identified by iTRAQ analysis. Gene expression profile of CsAPX1 encoding ascorbate peroxidase (APX) was regulated by light/dark conditions. AsA accumulation and APX activity were suppressed by light/dark conditions. SDS-PAGE analysis showed that the molecular mass of recombinant CsAPX1 protein was about 34.45 kDa. Subcellular localization indicated that CsAPX1 protein was a cytosol ascorbate peroxidase. Overexpression CsAPX1 in Arabidopsis indicated that the decrease of AsA content and APX activity in transgenic lines were less significant than that of WT during postharvest storage under light/dark conditions. These data suggested that CsAPX1 involved in regulating AsA metabolism through effecting on the changes of AsA accumulation and APX activity in fresh-cut tea leaves during postharvest storage under light/dark conditions.
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页数:14
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