Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes

被引：175

作者：

Lin, Sze Ki Carol ^{[1
]}

Du, Chenyu ^{[1
]}

Koutinas, Apostolis ^{[1
]}

Wang, Ruohang ^{[1
]}

Webb, Colin ^{[1
]}

机构：

[1] Univ Manchester, Sch Chem Engn & Analyt Sci, Satake Ctr Grain Proc Engn, Manchester M60 1QD, Lancs, England

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2008年 / 41卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

fermentation; product inhibition; substrate inhibition; growth kinetic; succinic acid; Actinobacillus succinogenes;

D O I：

10.1016/j.bej.2008.03.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The inhibition of substrate and products on the growth of Actinobacillus succinogenes in fermentation using glucose as the major carbon source was studied. A. succinogenes tolerated up to 143 g/L glucose and cell growth was completely inhibited with glucose concentration over 158g/L Significant decrease in succinic acid yield and prolonged lag phase were observed with glucose concentration above 100g/L Among the end-products investigated, formate was found to have the most inhibitory effect on succinic acid fermentation. The critical concentrations of acetate, ethanol, formate, pyruvate and succinate were 46, 42, 16,74,104 g/L, respectively. A growth kinetic model considering both substrate and product inhibition is proposed,which adequately simulates batch fermentation kinetics using both semi-defined and wheat-derived media. The model accurately describes the inhibitory kinetics caused by both externally added chemicals and the same chemicals produced during fermentation. This paper provides key insights into the improvement of succinic acid production and the modelling of inhibition kinetics. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：128 / 135

页数：8

共 29 条

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