Kinetochore-Microtubule Dynamics and Attachment Stability

被引：15

作者：

DeLuca, Jennifer G. ^{[1
]}

机构：

[1] Colorado State Univ, Dept Biochem & Mol Biol, Ft Collins, CO 80523 USA

来源：

MICROTUBULES: IN VIVO | 2010年 / 97卷

关键词：

SMALL-MOLECULE INHIBITOR; MITOTIC SPINDLE; KINESIN EG5; CHROMOSOME ALIGNMENT; MITOSIS; ORIENTATION; CONGRESSION; MECHANISMS; CELLS; FLUX;

D O I：

10.1016/S0091-679X(10)97004-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mitosis is the process by which a cell divides its genetic material equally into two daughter cells. Successful division requires that the two identical sister chromatids of a mitotic chromosome attach to the plus-ends of spindle microtubules (MTs) via their kinetochores, which are large protein structures built on centromeric DNA. Attachments between kinetochores and MTs must be persistent so that forces can be generated for chromosome movements, but at the same time they must be compliant, because attached MT plus-ends continuously polymerize and depolymerize to provide force for chromosome congression to the spindle equator. Both the attachment stability of kinetochore-MTs and the degree of dynamic instability exhibited by kinetochore-MTs must be precisely controlled to avoid errors in chromosome segregation. This chapter provides an overview of techniques used in cultured mammalian cells that measure stability and polymerization/depolymerization dynamics of kinetochore-MTs during mitosis.

引用

页码：53 / 79

页数：27

共 76 条

[1] Captivating capture: How microtubules attach to kinetochores [J].

Biggins, S ;

Walczak, CE .

CURRENT BIOLOGY, 2003, 13 (11) :R449-R460

[2] The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the, spindle checkpoint [J].

Biggins, S ;

Murray, AW .

GENES & DEVELOPMENT, 2001, 15 (23) :3118-3129

[3] COLD-LABILE AND COLD-STABLE MICROTUBULES IN MITOTIC SPINDLE OF MAMMALIAN-CELLS [J].

BRINKLEY, BR ;

CARTWRIGHT, J .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1975, 253 (JUN30) :428-439

[4] Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells [J].

Cameron, LA ;

Yang, G ;

Cimini, D ;

Canman, JC ;

Evgenieva, OK ;

Khodjakov, A ;

Danuser, G ;

Salmon, ED .

JOURNAL OF CELL BIOLOGY, 2006, 173 (02) :173-179

[5]

CASSIMERIS L, 1990, J CELL SCI, V96, P9

[6] TOGp Regulates Microtubule Assembly and Density During Mitosis and Contributes to Chromosome Directional Instability [J].

Cassimeris, Lynne ;

Becker, Bret ;

Carney, Bruce .

CELL MOTILITY AND THE CYTOSKELETON, 2009, 66 (08) :535-545

[7] Aneuploidy: a matter of bad connections [J].

Cimini, D ;

Degrassi, F .

TRENDS IN CELL BIOLOGY, 2005, 15 (08) :442-451

[8] Merotelic kinetochore orientation, aneuploidy, and cancer [J].

Cimini, Daniela .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2008, 1786 (01) :32-40

[9] Aurora kinase promotes turnover of kinetochore microtubules to reduce chromosome segregation errors [J].

Cimini, Daniela ;

Wan, Xiaohu ;

Hirel, Christophe B. ;

Salmon, E. D. .

CURRENT BIOLOGY, 2006, 16 (17) :1711-1718

[10]

DeBonis S, 2004, MOL CANCER THER, V3, P1079

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