Induction of stable RNA interference in mammalian cells

被引：56
作者：
Cullen, BR
机构：
[1] Duke Univ, Med Ctr, Ctr Virol, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
来源：
GENE THERAPY | 2006年 / 13卷 / 06期
关键词：
RNA interference; micro-RNAs; short hairpin RNAs; lentiviral vectors;
D O I：
10.1038/sj.gt.3302656
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knockdown of almost any vertebrate gene product in cultured cells or in experimental animals.
引用
收藏
页码：503 / 508
页数：6
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