Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential

被引：77
作者：
Rosowski, Kathryn A. [1 ]
Mertz, Aaron F. [3 ]
Norcross, Samuel [1 ]
Dufresne, Eric R. [3 ,4 ,5 ,6 ,7 ]
Horsley, Valerie [1 ,2 ]
机构：
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[2] Yale Univ, Dept Dermatol, New Haven, CT 06520 USA
[3] Yale Univ, Dept Phys, New Haven, CT 06520 USA
[4] Yale Univ, Dept Mech Engn, New Haven, CT 06520 USA
[5] Yale Univ, Dept Mat Sci, New Haven, CT 06520 USA
[6] Yale Univ, Dept Chem & Environm Engn, New Haven, CT 06520 USA
[7] Yale Univ, Dept Cell Biol, New Haven, CT 06520 USA
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
EXPRESSION; GROWTH; FORCES; PROLIFERATION; MORPHOGENESIS; INHIBITION; ADHESIONS; PATHWAY; TWIST;
D O I：
10.1038/srep14218
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In order to understand the mechanisms that guide cell fate decisions during early human development, we closely examined the differentiation process in adherent colonies of human embryonic stem cells (hESCs). Live imaging of the differentiation process reveals that cells on the outer edge of the undifferentiated colony begin to differentiate first and remain on the perimeter of the colony to eventually form a band of differentiation. Strikingly, this band is of constant width in all colonies, independent of their size. Cells at the edge of undifferentiated colonies show distinct actin organization, greater myosin activity and stronger traction forces compared to cells in the interior of the colony. Increasing the number of cells at the edge of colonies by plating small colonies can increase differentiation efficiency. Our results suggest that human developmental decisions are influenced by cellular environments and can be dictated by colony geometry of hESCs.
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页数：12
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