Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)
被引:63
作者:
Wilcox, Taylor M.
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Univ Montana, Div Biol Sci, Missoula, MT 59812 USA
US Forest Serv, Rocky Mt Res Stn, Missoula, MT USAUniv Montana, Div Biol Sci, Missoula, MT 59812 USA
Wilcox, Taylor M.
[1
,2
]
McKelvey, Kevin S.
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US Forest Serv, Rocky Mt Res Stn, Missoula, MT USAUniv Montana, Div Biol Sci, Missoula, MT 59812 USA
McKelvey, Kevin S.
[2
]
Young, Michael K.
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US Forest Serv, Rocky Mt Res Stn, Missoula, MT USAUniv Montana, Div Biol Sci, Missoula, MT 59812 USA
Young, Michael K.
[2
]
Lowe, Winsor H.
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机构:
Univ Montana, Div Biol Sci, Missoula, MT 59812 USAUniv Montana, Div Biol Sci, Missoula, MT 59812 USA
Lowe, Winsor H.
[1
]
Schwartz, Michael K.
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USDA, Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Missoula, MT USAUniv Montana, Div Biol Sci, Missoula, MT 59812 USA
Schwartz, Michael K.
[3
]
机构:
[1] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA
[2] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA
[3] USDA, Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Missoula, MT USA
Environmental DNA (eDNA) sampling has become a widespread approach for detecting aquatic animals with high potential for improving conservation biology. However, little research has been done to determine the size of particles targeted by eDNA surveys. In this study, we conduct particle distribution analysis of eDNA from a captive Brook Trout (Salvelinus fontinalis) in a flow-through mesocosm. Our data suggest that 1.2-10-mu m particles are the most common size of eDNA from Brook Trout, which is consistent with our hypotheses that eDNA in the environment is comprised of loosely aggregated smaller particles, resulting in high inter-sample heterogeneity. These findings are similar to those of a study on Common Carp (Cyprinus carpio) in lentic systems.
机构:
Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USAVirginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA
Densmore, CL
Smith, SA
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机构:Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA
Smith, SA
Holladay, SD
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机构:Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA
机构:
Fisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Cote, D.
Tibble, B.
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Tulloch Environm, 1942 Regent St, Sudbury, ON P3E 5V5, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Tibble, B.
Curry, R. A.
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机构:
Univ New Brunswick, Canadian Rivers Inst, Biol Dept, Fredericton, NB E3B 5A3, Canada
Univ New Brunswick, Fac Forestry & Environm Management, Fredericton, NB E3B 5A3, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Curry, R. A.
Peake, S.
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机构:
Univ New Brunswick, Canadian Rivers Inst, Biol Dept, Fredericton, NB E3B 5A3, Canada
Univ New Brunswick, Fac Forestry & Environm Management, Fredericton, NB E3B 5A3, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Peake, S.
Adams, B. K.
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Govt Newfoundland & Labrador, Wildlife Div, Dept Fisheries & Land Resources, 192 Wheelers Rd, Corner Brook, NF A2H 7S1, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Adams, B. K.
Clarke, K. D.
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Fisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
Clarke, K. D.
Perry, R.
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机构:
Govt Newfoundland & Labrador, Wildlife Div, Dept Fisheries & Land Resources, 192 Wheelers Rd, Corner Brook, NF A2H 7S1, CanadaFisheries & Oceans Canada, 80 East White Hills Rd, St John, NF A1C 5X1, Canada
机构:
Fed Agcy Water Management, Inst Water Ecol Fisheries & Lake Res, Scharfling 18, A-5310 Mondsee, Austria
Fishfarm Kreuzstein, Unterach, AustriaFed Agcy Water Management, Inst Water Ecol Fisheries & Lake Res, Scharfling 18, A-5310 Mondsee, Austria
机构:
Trent Univ, Environm & Life Sci Grad Program, Peterborough, ON, Canada
Canada Ctr Inland Waters, Fisheries & Oceans Canada, Burlington, ON, CanadaTrent Univ, Environm & Life Sci Grad Program, Peterborough, ON, Canada
Blair, Scott G.
Gutowsky, Lee F. G.
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机构:
Trent Univ, Environm & Life Sci Grad Program, Peterborough, ON, Canada
Ontario Minist Nat Resources & Forestry, Aqut Res & Monitoring Sect, Peterborough, ON, CanadaTrent Univ, Environm & Life Sci Grad Program, Peterborough, ON, Canada
Gutowsky, Lee F. G.
Fox, Michael G.
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Trent Univ, Sch Environm, 2140 East Bank Dr, Peterborough, ON K9L 1Z8, Canada
Trent Univ, Dept Biol, 2140 East Bank Dr, Peterborough, ON K9L 1Z8, CanadaTrent Univ, Environm & Life Sci Grad Program, Peterborough, ON, Canada