Polymerase Recognition of a Watson-Crick-Like Metal-Mediated Base Pair: Purine-2,6-Dicarboxylate•Copper(II)•Pyridine

被引：44
作者：
Kim, Eun-Kyong [1 ]
Switzer, Christopher [1 ]
机构：
[1] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA
来源：
CHEMBIOCHEM | 2013年 / 14卷 / 18期
基金：
美国国家航空航天局; 美国国家科学基金会;
关键词：
base pairs; DNA polymerases; DNA structures; metal-mediated base pairs; synthetic biology; NUCLEIC-ACIDS; DNA-DUPLEX; NUCLEOTIDES; EFFICIENT; AMPLIFICATION; DEPROTECTION; REPLICATION; NUCLEOSIDES; SYSTEMS; ESTERS;
D O I：
10.1002/cbic.201300634
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new beginning: A Cu2+-mediated Watson-Crick-like base pair was recognized by nine out of ten DNA polymerases studied. The purine-2,6- dicarboxylate×Cu2+×3-pyridine base pair was formed in the presence Cu2+ ions, but not in the absence of copper. It is concluded that metal-ion mediated bonding interactions are sufficient for non-canonical base-pair synthesis by enzymes. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：2403 / 2407
页数：5
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