Polymerase Recognition of a Watson-Crick-Like Metal-Mediated Base Pair: Purine-2,6-Dicarboxylate•Copper(II)•Pyridine

被引：44

作者：

Kim, Eun-Kyong ^{[1
]}

Switzer, Christopher ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

来源：

CHEMBIOCHEM | 2013年 / 14卷 / 18期

基金：

美国国家科学基金会; 美国国家航空航天局;

关键词：

base pairs; DNA polymerases; DNA structures; metal-mediated base pairs; synthetic biology; NUCLEIC-ACIDS; DNA-DUPLEX; NUCLEOTIDES; EFFICIENT; AMPLIFICATION; DEPROTECTION; REPLICATION; NUCLEOSIDES; SYSTEMS; ESTERS;

D O I：

10.1002/cbic.201300634

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new beginning: A Cu2+-mediated Watson-Crick-like base pair was recognized by nine out of ten DNA polymerases studied. The purine-2,6- dicarboxylate×Cu2+×3-pyridine base pair was formed in the presence Cu2+ ions, but not in the absence of copper. It is concluded that metal-ion mediated bonding interactions are sufficient for non-canonical base-pair synthesis by enzymes. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2403 / 2407

页数：5

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