Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus

被引：16

作者：

Fujinami, Daisuke ^{[1
]}

Matsumoto, Masaki ^{[2
]}

Noguchi, Takuya ^{[3
]}

Sonomoto, Kenji ^{[3
,4
]}

Kohda, Daisuke ^{[1
,5
]}

机构：

[1] Kyushu Univ, Med Inst Bioregulat, Div Struct Biol, Multiscale Res Ctr Med Sci, Fukuoka 8128582, Japan

[2] Kyushu Univ, Med Inst Bioregulat, Div Cell Biol, Dept Mol & Cellular Biol, Fukuoka 8128582, Japan

[3] Kyushu Univ, Div Appl Mol Microbiol & Biomass Chem, Dept Biosci & Biotechnol, Lab Microbial Technol,Fac Agr,Grad Sch, Fukuoka 8128581, Japan

[4] Kyushu Univ, Dept Funct Metab Design, Bioarchitecture Ctr, Lab Funct Food Design, Fukuoka 8128581, Japan

[5] Kyushu Univ, Med Inst Bioregulat, Res Ctr Adv Immunol, Fukuoka 8128582, Japan

来源：

CARBOHYDRATE RESEARCH | 2014年 / 387卷

关键词：

Pyrococcus furiosus; Archaeal N-glycan structure; Enzymatic preparation; Oligosaccharyltransferase; NMR; MS; S-LAYER GLYCOPROTEIN; PROTEIN GLYCOSYLATION; OLIGOSACCHARYLTRANSFERASE;

D O I：

10.1016/j.carres.2014.01.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An oligosaccharide chain attached to the asparagine residue in a structurally defined peptide was produced by an in vitro oligosaccharide-transfer reaction, using membrane fractions that contained the oligosaccharyltransferase from the hyperthermophilic archaeon, Pyrococcus furiosus. The chemical structure of the N-glycan was elucidated by sugar analysis, NMR spectroscopy, and MS spectrometry, which revealed the structure. alpha-D-ManpNAc-(1 -> 4)-alpha-D-GlcAp-(1 -> 3)-alpha-D-Manp-(1 -> 2)-alpha-D-Manp-(1 -> 3)-beta-D-GalpNAc-Asm 3 2 up arrow up arrow 1 1 beta-D-Xylp beta-D-Xylp The shorter glycan structures lacking one or two xylose residues were also transferred by the P. furiosus oligosaccharyltransferase. The archaeal N-glycans are known to exhibit a high degree of structural variation. The structure of the P. furiosus N-glycan is novel and unique. The present data will be useful for structural and functional studies of the P. furiosus oligosaccharyltransferase. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：30 / 36

页数：7

共 21 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].