Transglycosylation of stevioside by cyclodextrin glucanotransferases of various groups of microorganisms

被引：46

作者：

Abelyan, VA ^{[1
]}

Balayan, AM

Ghochikyan, VT

Markosyan, AA

机构：

[1] Natl Acad Sci Armenia, Inst Microbiol, Abovyan 375510, Armenia

[2] Stevian Biotechnol Corp, Kuala Lumpur 50450, Malaysia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2004年 / 40卷 / 02期

关键词：

D O I：

10.1023/B:ABIM.0000018914.08571.50

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside (in order to remove bitterness and aftertaste), with cyclodextrins (CDs) being used as donors. It was shown that CGTases produced by extremophilic microorganisms are effective biocatalysts. Optimum temperature and pH of these enzymes were 45degreesC and pH 6.5-7.5, respectively. The optimum stevioside-to-CD ratio and total concentration of dry matter for the synthesis of the best-tasting product were 1 : 1 (w/w) and 11.6%, respectively.

引用

页码：129 / 134

页数：6

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