Synthesis of isopropyl-1-Thio-beta-D-glucopyranoside (IPTGlc), an inducer of Aspergillus niger B1 beta-glucosidase production

被引:14
作者
Birk, R
Ikan, A
Bravdo, B
Braun, S
Shoseyov, O
机构
[1] HEBREW UNIV JERUSALEM,FAC AGR,KENNEDY LEIGH CTR HORT RES,IL-76100 REHOVOT,ISRAEL
[2] HEBREW UNIV JERUSALEM,FAC AGR,OTTO WARBURG CTR AGR BIOTECHNOL,IL-76100 REHOVOT,ISRAEL
[3] HEBREW UNIV JERUSALEM,ALEXANDER SILBERMAN INST LIFE SCI,DEPT BIOL CHEM,IL-91904 JERUSALEM,ISRAEL
关键词
Aspergillus niger; beta-glucosidase; isopropyl-1-thio-beta-D-glucopyranoside; induction;
D O I
10.1007/BF02788804
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Production of beta-glucosidase in Aspergillus niger B1 is subjected to catabolic repression by glucose. Aspergillus niger B1 grown on bran as a carbon source secreted beta-glucosidase. The maximum level of the enzyme was reached after 7 d of fermentation. Addition of 1% glucose to the medium suppressed beta-glucosidase production to undetectable levels. In this study, the organic synthesis of a potential inducer of beta-glucosidase production by A. niger B1's reported. Isopropyl-1-thio-beta-D-glucopyranoside (IPTGlc) was synthesized using a two-step organic synthesis protocol. The H-NMR data agreed with those reported previously for the galactoside analog. When IPTGlc was added 24 h after inoculation at a final concentration of 0.4 mM, similar levels of beta-glucosidase were reached 3 to 4 d earlier as compared to fermentation without IPTGlc induction. In practice, this may translate to a more efficient method of producing beta-glucosidase from this fungus.
引用
收藏
页码:25 / 30
页数:6
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