An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

被引：12
作者：
Blatzer, Michael [1 ]
Gsaller, Fabio [1 ]
Abt, Beate [1 ]
Schrettl, Markus [1 ,2 ]
Specht, Thomas [2 ]
Haas, Hubertus [1 ]
机构：
[1] Bioctr, Div Mol Biol, Christian Doppler Lab Fungal Biotechnol, A-6020 Innsbruck, Austria
[2] Sandoz GmbH, A-6250 Kundl, Austria
来源：
JOURNAL OF BIOTECHNOLOGY | 2014年 / 169卷
关键词：
Cephalosporin; Inducible promoter; Xylanase; Xylan/xylose-dependent gene expression; Selection marker recycling; CEPHALOSPORIN-C BIOSYNTHESIS; ASPERGILLUS-NIDULANS; FILAMENTOUS FUNGI; SYSTEM; TRANSFORMATION; REPRESSION; MARKER;
D O I：
10.1016/j.jbiotec.2013.11.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1(P), is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1(P)-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1(P) promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：82 / 86
页数：5
相关论文
共 31 条
[1]   PSI-BLAST pseudocounts and the minimum description length principle [J].
Altschul, Stephen F. ;
Gertz, E. Michael ;
Agarwala, Richa ;
Schaffer, Alejandro A. ;
Yu, Yi-Kuo .
NUCLEIC ACIDS RESEARCH, 2009, 37 (03) :815-824
[2]   Evolution of β-lactam biosynthesis genes and recruitment of trans-acting factors [J].
Brakhage, AA ;
Al-Abdallah, Q ;
Tüncher, A ;
Spröte, P .
PHYTOCHEMISTRY, 2005, 66 (11) :1200-1210
[3]   HgrA is necessary and sufficient to drive hyphal growth in the dimorphic pathogen Penicillium marneffei [J].
Bugeja, Hayley E. ;
Hynes, Michael J. ;
Andrianopoulos, Alex .
MOLECULAR MICROBIOLOGY, 2013, 88 (05) :998-1014
[4]   2 DIFFERENT, ADJACENT AND DIVERGENT ZINC-FINGER BINDING-SITES ARE NECESSARY FOR CREA-MEDIATED CARBON CATABOLITE REPRESSION IN THE PROLINE GENE-CLUSTER OF ASPERGILLUS-NIDULANS [J].
CUBERO, B ;
SCAZZOCCHIO, C .
EMBO JOURNAL, 1994, 13 (02) :407-415
[5]   A homologue of the Aspergillus velvet gene regulates both cephalosporin C biosynthesis and hyphal fragmentation in Acremonium chrysogenum [J].
Dreyer, Jacqueline ;
Eichhorn, Heiko ;
Friedlin, Ernst ;
Kuernsteiner, Hubert ;
Kueck, Ulrich .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (10) :3412-3422
[6]   THE ETHANOL UTILIZATION REGULON OF ASPERGILLUS-NIDULANS - THE ALCA-ALCR SYSTEM AS A TOOL FOR THE EXPRESSION OF RECOMBINANT PROTEINS [J].
FELENBOK, B .
JOURNAL OF BIOTECHNOLOGY, 1991, 17 (01) :11-17
[7]   The first promoter for conditional gene expression in Acremonium chrysogenum: Iron starvation-inducible mir1P [J].
Gsaller, Fabio ;
Blatzer, Michael ;
Abt, Beate ;
Schrettl, Markus ;
Lindner, Herbert ;
Haas, Hubertus .
JOURNAL OF BIOTECHNOLOGY, 2013, 163 (01) :77-80
[8]   Homologous recombination in the antibiotic producer Penicillium chrysogenum: strain ΔPcku70 shows up-regulation of genes from the HOG pathway [J].
Hoff, Birgit ;
Kamerewerd, Jens ;
Sigl, Claudia ;
Zadra, Ivo ;
Kueck, Ulrich .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) :1081-1094
[9]   Loss of glucose repression in an Acremonium chrysogenum β-lactam producer strain and its restoration by multiple copies of the cre1 gene [J].
Jekosch, K ;
Kück, U .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 54 (04) :556-563
[10]   Application of the Saccharomyces cerevisiae FLP/FRT Recombination System in Filamentous Fungi for Marker Recycling and Construction of Knockout Strains Devoid of Heterologous Genes [J].
Kopke, Katarina ;
Hoff, Birgit ;
Kueck, Ulrich .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (14) :4664-4674
1234